Joseph Sambrook

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Joseph Frank "Joe" Sambrook (born March  1, 1939 in Liverpool ; † June 14, 2019 ) was a British - Australian molecular biologist and cancer researcher . From 1995 he was a professor at the University of Melbourne and worked, among other things, on oncoviruses , the molecular basis of carcinogenesis and the transport and folding of proteins . In recognition of his research achievements he was among other things in the Royal Society and theAustralian Academy of Science admitted.

Life

Joseph Sambrook was born in Liverpool in 1939 and completed his academic training at the University of Liverpool , where he received a BSc in microbiology in 1962 , and at the Australian National University , where he did a thesis on animal virus genetics four years later PhD . He then worked at the Laboratory of Molecular Biology of the Medical Research Council in Cambridge from 1966/1967 and from 1967 to 1969 at the Salk Institute for Biological Studies as a post-doctoral student in La Jolla .

From 1969 to 1985 he worked as a research group leader at the Cold Spring Harbor Laboratory (CSHL) in Cold Spring Harbor , New York State . He then moved to the Southwestern Medical Center of the University of Texas in Dallas , where he was Professor and Head of the Department of Biochemistry from 1985 to 1991, as well as the Burgher Center for Molecular Cardiology from 1986 to 1993 and the McDermott from 1991 to 1994 Center for Human Growth and Development . From 1995 he was Director of Research at the Peter MacCallum Cancer Institute in Melbourne and Professor in the Department of Pathology at the University of Melbourne .

Joseph Sambrook was married to the biochemist Mary-Jane Gething and had one daughter.

Scientific work

Joseph Sambrook's research focused on oncoviruses such as SV-40 and adenoviruses , molecular biological and genetic aspects of the development of cancer as well as protein transport and protein folding in cells .

During his time at CSHL in 1973, he described the use of agarose and ethidium bromide for gel electrophoresis and the detection of DNA . In 1988 he and his team at Southwestern Medical Center discovered the Unfolded Protein Response , a response of cells to stress caused by the accumulation of incorrectly folded proteins .

In addition, with the work "Molecular Cloning: A Laboratory Manual" he founded a laboratory manual on cloning methods , which is considered the worldwide reference standard on this topic.

Awards

Joseph Sambrook was a member of the Royal Society from 1985 and of the Australian Academy of Science from 2000 . At the CSHL, the Joseph F. Sambrook Laboratory is a laboratory building named after him. His alma mater, the University of Liverpool, awarded him an honorary doctorate in 2007 .

Works (selection)

  • DNA microarrays: A Molecular Cloning Manual. Cold Spring Harbor 2003
  • Molecular Cloning: A Laboratory Manual. Fourth edition. Cold Spring Harbor 2012 (three volumes)
  • Phillip A. Sharp, Bill Sugden, Joe Sambrook: Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose-ethidium bromide electrophoresis. In: Biochemistry . 12 (16 )/1973. ACS Publications, pp. 3055-3063, ISSN  0006-2960
  • Yasunori Kozutsumi, Mark Segal, Karl Normington, Mary-Jane Gething, Joe Sambrook: The presence of malfolded proteins in the endoplasmic reticulum signals the induction of glucose-regulated proteins. In: Nature . Volume 332, Edition 6163, March 31, 1988, pp. 462-464

Web links

personal data
SURNAME Sambrook, Joseph
ALTERNATIVE NAMES Sambrook, Joseph Frank (full name)
BRIEF DESCRIPTION British-Australian molecular biologist and cancer researcher
DATE OF BIRTH March 1, 1939
PLACE OF BIRTH Liverpool
DATE OF DEATH June 14, 2019