Pappenheim staining
With the Panoptic staining according to Pappenheim (named after Artur Pappenheim , 1870-1916) air-dried blood smears are treated with several color solutions. These solutions contain, among other things, stabilizers ( glycerine ), fixatives ( methanol ), acidic and basic dyes . The Pappenheim staining is a combination of the May-Grünwald staining and the Giemsa staining .
Basic dyes such as methylene blue and brilliant cresyl blue form active dye components with a positive charge in aqueous solution and stain cell components with a negative charge a bluish tinge. Acid dyes such as eosin and acid fuchsin break down in aqueous solution into active dye components with a negative charge and color protein structures with a positive charge. Neutrophils can be stained with both basic and acidic dyes.
The Pappenheim staining is a panoptic / panchromatic staining, since basophilic, neutrophilic and eosinophilic structures are shown.
Result
![](https://upload.wikimedia.org/wikipedia/commons/thumb/9/92/Pappelheimfaerbung.jpeg/220px-Pappelheimfaerbung.jpeg)
The various cellular and humoral (liquid) components of the blood can be seen in different colors under the light microscope .
Blood component | Coloring |
---|---|
Erythrocytes | pink |
Nuclei of leukocytes and nucleated erythrocytes | red-violet |
Eosinophil granules | brick-red to red-brown |
Basophilic granules | dark purple to black |
Neutrophil granules | light purple |
Cytoplasm of lymphocytes | Light Blue |
Monocyte plasma | gray-blue |
literature
- Maria Mulisch, Ulrich Welsch (ed.): Romeis - microscopic technology . 19th edition. Springer Spectrum, Berlin, Heidelberg 2015, ISBN 978-3-642-55189-5 .