Trichrome staining
![](https://upload.wikimedia.org/wikipedia/commons/thumb/f/fd/Dilated_peri-tubular_capillaries_filled_with_sickled_RBCs%2C_original_Gomori%27s_trichrome_stain.jpg/220px-Dilated_peri-tubular_capillaries_filled_with_sickled_RBCs%2C_original_Gomori%27s_trichrome_stain.jpg)
Dilated peritubular capillary vessels with sickle cells, stained according to Gömöri.
The trichrome staining is a method for staining cells and tissues with three dyes (chromophores).
principle
The name trichrome is derived from the use of three dyes, for example the MSB trichrome stain ( Ponceau 6R together with Martinsgelb and water blue ). The specimens to be stained are fixed before histological staining , which stabilizes the structure of the cells and prevents autolysis . Various fixatives are used for this. B. a ten percent neutral buffered formaldehyde solution or the Bouin solution . Excess fixing agents are then washed out with a buffer if the staining requires this pretreatment.
variants
The variants of the trichrome staining are sorted chronologically.
- Van Gieson trichrome stain
- Mallory trichrome stain
- Masson trichrome stain
- Masson-Goldner trichrome stain
- Masson-Lillie trichrome stain
- Gömöri trichrome staining
- Wheatley trichrome stain
- ASTRIN trichrome stain
- Raţ trichrome stain
Web links
- Masson's Trichrome Protocol
- Bryan D. Llewellyn: Differential Staining With Acid Dyes. PDF .
Individual evidence
- ↑ KV Kanodia, AV Vanikar, KR Goplani, SB Gupta, HL Trivedi: Sickle cell nephropathy with diffuse proliferative lupus nephritis: a case report . In: Diagn Pathol . tape 3 , 2008, p. 9 , doi : 10.1186 / 1746-1596-3-9 , PMID 18307766 , PMC 2275218 (free full text).
- ↑ Ira van Gieson: Laboratory notes of technical methods for the nervous system. In: NY Med J. (1889), Volume 50, p. 57.
- ^ I. Singh: A simple substitute of iron haematoxylin in the van Gieson and Masson trichrome procedures. In: Anatomischer Anzeiger . Volume 124, Number 5, 1969, ISSN 0003-2786 , pp. 490-491. PMID 4185232 .
- ^ FB Mallory: A Contribution to staining methods. I. A differential stain for connective tissue fibril and reticulum. II. Chloride of Iron Haematoxylin for nuclei and fibrin. In: J Exp Med (1900), Volume 5, Issue 1, pp. 15-20. PMC 2117995 (free full text).
- ↑ Maria Wołuń-Cholewa, Krzysztof Szymanowski, Mirosław Andrusiewicz, Anna Szczerba, Jerzy B. Warchoł: Trichrome Mallory's stain may indicate differential rates of RNA synthesis in eutopic and ectopic endometrium. In: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society. Volume 48, Number 1, January 2010, ISSN 1897-5631 , pp. 148-152, doi: 10.2478 / v10042-008-0106-4 , PMID 20529831 .
- ^ P. Masson: Some histological methods; trichrome stainings and their preliminary technique. In: J. Tech. Methods. (1929), Vol. 12, pp. 75-90.
- ↑ J. Ouyang, M. Guzman, F. Desoto-Lapaix, MR Pincus, R. Wieczorek: Utility of desmin and a Masson's trichrome method to detect early acute myocardial infarction in autopsy tissues. In: International journal of clinical and experimental pathology. Volume 3, Number 1, 2009, ISSN 1936-2625 , pp. 98-105. PMID 19918333 , PMC 2776270 (free full text).
- ↑ Jacques Goldner: A modification of the masson trichrome technique for routine laboratory purposes. In: Am J Pathol. (1938), Volume 14, Issue 2, pp. 237-243. PMC 1964940 (free full text).
- ^ Y. Wang, P. Spencer: Evaluation of the interface between one-bottle adhesive systems and dentin by Goldner's trichrome. In: American journal of dentistry. Volume 18, Number 1, February 2005, ISSN 0894-8275 , pp. 66-72. PMID 15810485 .
- ^ RD Lillie: Studies on selective staining of collagen with acid aniline dyes. In: J. Tech. Meth. (1945), Vol. 25, pp. 1-47.
- ↑ G. Gömöri: A rapid one-step trichrome stain. In: American journal of clinical pathology. Volume 20, Number 7, July 1950, ISSN 0002-9173 , pp. 661-664. PMID 15432364 .
- ^ Y. Harati, K. Matta: Gomori's trichrome stain. In: Archives of neurology. Volume 36, Number 7, July 1979, ISSN 0003-9942 , pp. 454-455. PMID 88216 .
- ^ WB Wheatley: A rapid staining procedure for intestinal amoebae and flagellates. In: American journal of clinical pathology. Volume 21, Number 10, October 1951, ISSN 0002-9173 , pp. 990-991. PMID 14885115 .
- Jump up ↑ DJ Flournoy, SJ McNabb, ED Dodd, MH Shaffer: Rapid trichrome stain. In: Journal of clinical microbiology. Volume 16, Number 3, September 1982, ISSN 0095-1137 , pp. 573-574. PMID 6182159 , PMC 272416 (free full text).
- ↑ FM Salleh, TS Anuar, AM Yasin, N. Moktar: Wintergreen oil: a novel method in Wheatley's trichrome staining technique. In: Journal of microbiological methods. Volume 91, Number 1, October 2012, ISSN 1872-8359 , pp. 174-178, doi: 10.1016 / j.mimet.2012.08.004 , PMID 22986100 .
- ↑ G. Balogh, P. Kovács, G. Csaba: ASTRIN - a new trichrome staining technique. In: Journal for microscopic-anatomical research. Volume 96, Number 1, 1982, ISSN 0044-3107 , pp. 138-144. PMID 6179312 .
- ↑ M. Raica, O. Mederle, G. Raţ: A new trichrome staining method: its practice and application in normal tissues. In: Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie. Volume 44, Numbers 1-4, 1998 Jan-Dec, pp. 3-6, ISSN 1220-0522 . PMID 15678835 .