Vital staining

Vital staining with methylene blue: cells of the human oral mucosa

Among vital staining refers to the staining of cells or cell organelles, and their structure and function, in contrast to Letalfärbung is not impaired.

function

With vital staining, especially elements in the microscopic range (cells, their organelles , etc.) can be made visible, since under normal circumstances they are mostly not or only poorly recognizable in the light microscope . The advantage of this staining is that the function and structure of the stained cell is not destroyed or changed because the dye is membrane permeable , for example .

Examples

• Brilliant cresyl blue
• Giemsa stain
• Methylene blue
• Neutral red
• Toluidine blue staining
• Triphenyl tetrazolium chloride
• Acid Violet 17

swell

• Chromoendoskopie.de: Dyes: methylene blue ( Memento from September 29, 2007 in the Internet Archive )

See also

• Cell viability

Individual evidence

1. ↑ A. Tura, A. Alt, J. Lüke, S. Grisanti, C. Haritoglou, CH Meyer, K. Nassar, M. Lüke: Biocompatibility of the vital dye Acid Violet-17 on retinal pigment epithelial cells. In: Clinical ophthalmology (Auckland, NZ). Volume 10, 2016, pp. 1435-1445, doi : 10.2147 / OPTH.S105695 , PMID 27536056 , PMC 4975140 (free full text).