Metabolomics: Difference between revisions
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Added comment about FT-ICR-MS under MS sub-head, and deleted listing of FTIR as a 'key technique'. FTIR allows 'metabolic fingerprinting' but does not ID individual metabolites. |
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'''Metabolomics''' is the study of the metabolic profile of a given cell, tissue, fluid, organ or organism at a given point in time. The [[metabolome]] represents the end products of gene expression. While mRNA [[gene expression]] data and [[proteomics|proteomic]] analyses hint at what might be happening in a cell, [[metabolite]]s and |
'''Metabolomics''' is the study of the metabolic profile of a given cell, tissue, fluid, organ or organism at a given point in time. The [[metabolome]] represents the end products of gene expression. While mRNA [[gene expression]] data and [[proteomics|proteomic]] analyses hint at what might be happening in a cell, [[metabolite]]s and their relative accumulation can indicate what is happening that cell. |
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===Key technologies=== |
===Key technologies=== |
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*[[Mass spectrometry]] particularly gas chromatography mass spectrometry (GC MS), and liquid chromatography mass spectrometry (LC MS). |
*[[Mass spectrometry]] particularly gas chromatography mass spectrometry (GC MS), and liquid chromatography mass spectrometry (LC MS). In addition, direct-infusion mass spectrometry is becoming increasingly popular, especially for high-resolution techniques such as Fourier-transform ion-cyclotron-resonance mass spectrometry (FT-ICR-MS). |
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*[[Fourier transform spectroscopy|Fourier-transform]] [[infrared spectroscopy]]. |
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*High pressure liquid [[chromatography]] (HPLC). |
*High pressure liquid [[chromatography]] (HPLC). |
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*[[Nuclear magnetic resonance]] (NMR) spectrometry |
*[[Nuclear magnetic resonance]] (NMR) spectrometry |
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Revision as of 17:29, 15 October 2004
Metabolomics is the study of the metabolic profile of a given cell, tissue, fluid, organ or organism at a given point in time. The metabolome represents the end products of gene expression. While mRNA gene expression data and proteomic analyses hint at what might be happening in a cell, metabolites and their relative accumulation can indicate what is happening that cell.
Key technologies
- Mass spectrometry particularly gas chromatography mass spectrometry (GC MS), and liquid chromatography mass spectrometry (LC MS). In addition, direct-infusion mass spectrometry is becoming increasingly popular, especially for high-resolution techniques such as Fourier-transform ion-cyclotron-resonance mass spectrometry (FT-ICR-MS).
- High pressure liquid chromatography (HPLC).
- Nuclear magnetic resonance (NMR) spectrometry
See also: proteomics, glycomics