Alabama and Florida Railroad
Alabama and Florida Railroad or Alabama and Florida Railway stands for:
- Alabama and Florida Railroad (1836–1868) "of Alabama" , a predecessor of the Mobile and Montgomery Railroad
- Alabama and Florida Railroad (1853–1872) "of Florida" , a predecessor of the Pensacola and Louisville Railroad
- Alabama and Florida Railroad (1898-1900) , a predecessor of the Louisville and Nashville Railroad (Georgiana – Graceville railway line)
- Alabama and Florida Railroad (1937-1941) , a successor to the Alabama, Florida and Gulf Railroad
- Alabama and Florida Railroad (1986-1992) , a short-line railroad company (Georgiana – Graceville railroad)
- Alabama and Florida Railway , a short-line railroad company (Georgiana – Graceville Railway , 1992–2011)