Lawns of bacteria
As a lawn of bacteria is known in microbiology a dense sowing of bacteria on a solid growth medium (usually a agar plate ). For this purpose, a pure culture of a bacterial strain is evenly distributed on the plate with an inoculation loop or a Drigalski spatula and usually incubated overnight. Bacterial growth is evenly distributed on the agar plate.
The technique of the bacterial lawn is used in microbiological diagnostics to determine the presence of inhibitors such. B. to prove with the help of the inhibition zone test or to examine the sensitivity of a certain bacterial strain to different antibiotics ( antibiogram ). In the inhibition zone test, the bacterial lawn usually consists of a pure culture of Bacillus subtilis (in medical diagnostics) or Serratia marcescens (as a bio-indicator in environmental biological investigations). In both test methods, after the bacterial lawn has been spread out, a piece of paper with the sample or antibiotic to be tested is placed on it. After incubation, a zone of inhibition of different sizes indicates an inhibition of bacterial growth.
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- Betty A. Forbes et al .: Bailey & Scott's Diagnostic Microbiology . 12th edition 2007 ISBN 0-323-03065-3