Bio-layer interferometry

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The bio-layer interferometry measures the binding of molecules to each other on the basis of the change in refractive index upon binding. It is a labelless method for determining protein-protein interactions .

principle

Bio-Layer interferometry measures the refractive index before and after molecules bind to molecules that have previously been coated on a surface. The bond increases the layer thickness on the surface, which changes the refractive index. The method, like surface plasmon resonance spectroscopy , requires comparatively high concentrations of the molecules to be examined, but can also be carried out with unpurified samples.

Individual evidence

  1. ^ MA Cooper: Current Biosensor Technologies in Drug Discovery. In: Drug Discovery World (2006), pp. 68-82. PDF .
  2. ↑ RL Rich, DG Myszka: Higher-throughput, label-free, real-time molecular interaction analysis. In: Analytical biochemistry. Volume 361, Number 1, February 2007, pp. 1-6, ISSN  0003-2697 . doi : 10.1016 / year from 2006.10.040 . PMID 17145039 .
  3. ^ Y. Fang: Label-free Optical Biosensors in Drug Discovery. In: Trends in Biopharmaceutical Industry (2007), Volume 3, pp. 34–38 PDF ( Memento of the original from January 8, 2014 in the Internet Archive ) Info: The archive link was inserted automatically and has not yet been checked. Please check the original and archive link according to the instructions and then remove this notice. . @1@ 2Template: Webachiv / IABot / tbiweb.org
  4. ↑ J. Fransson, A. Teplyakov, G. Raghunathan, E. Chi, W. Cordier, T. Dinh, Y. Feng, J. Giles-Komar, G. Gilliland, B. Lollo, TJ Malia, W. Nishioka, G. Obmolova, S. Zhao, Y. Zhao, RV Swanson, JC Almagro: Human framework adaptation of a mouse anti-human IL-13 antibody. In: Journal of molecular biology. Volume 398, Number 2, April 2010, pp. 214-231, ISSN  1089-8638 . doi : 10.1016 / j.jmb.2010.03.004 . PMID 20226193 .
  5. ↑ Y. Abdiche, D. Malashock, A. Pinkerton, J. Pons: Determining kinetics and affinities of protein interactions using a parallel real-time label-free biosensor, the Octet. In: Analytical biochemistry. Volume 377, Number 2, June 2008, pp. 209-217, ISSN  1096-0309 . doi : 10.1016 / year from 2008.03.035 . PMID 18405656 .