Dot blot
A dot blot is a biochemical method for the qualitative detection of membrane-immobilized molecules by immunostaining or with a gene probe .
principle
A sample is dropped onto a membrane made of nitrocellulose or PVDF or onto filter paper and then often dried. After the adsorption of the molecules in the sample to the membrane, the membrane is blocked and single molecular species selectively by immunostaining detected. The principle of the dot blot is similar to the Western blot for proteins or the Southern blot for DNA and the Northern blot for RNA , but without an electrophoretic separation of the molecules in the sample. The dot blot can therefore be carried out comparatively faster, but does not allow the molar mass of the molecule to be detected to be determined and therefore cannot identify any false-positive immunostaining.
literature
- FC Kafatos, CW Jones, A. Efstratiadis: Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. In: Nucleic acids research. Volume 7, Number 6, November 1979, pp. 1541-1552, PMID 503860 , PMC 342326 (free full text).
Individual evidence
- ↑ Monika Jansohn: genetic engineering methods. Springer-Verlag, 2012, ISBN 978-3-827-42430-3 , pp. 289, 310.
- ↑ Robert E. Farrell, Jr .: RNA Methodologies. Academic Press, 2010, ISBN 978-0-080-45476-4 , pp. 179-189.