Hair perforation test

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Positive hair perforation test (pathogen: Trichophyton mentagrophytes )

The hair perforation test is a detection method for the detection of certain dermatophytes that are able to form perforating (penetrating) organs on hair. It is mainly used to detect dermatophytoses caused by zoophilic dermatophytes such as Trichophyton mentagrophytes .

For the hair perforation test, blonde, fine human hair is cut into pieces about 3 cm long, autoclaved and placed in Petri dishes with sterile water. Then a few drops of an aqueous yeast extract solution are added and finally mycelium from a pure culture of the dermatophyte to be examined. After 2 to 4 weeks, if the detection is positive, cone-shaped or wedge-shaped cavities, the organs of hair perforation, are formed, which can be detected with a light microscope.

literature

  • Barbara Beifuß: Establishment of an immediate molecular diagnosis of dermatophytes at the species level from native material from patients with skin mycosis using polymerase chain reaction (PCR) and real-time PCR (LightCycler). Diss. Ludwig Maximilians University Munich 2008. ( full text ; PDF; 1.5 MB)