Lipidomics

Classes of biogenic lipids

In biochemistry, lipidomics refers to research into the investigation of lipids . Lipidomics is a branch of metabolomics .

properties

In lipidomics, systems biology methods of high throughput and bioinformatics are used, but also mass spectrometry , NMR spectroscopy , fluorescence spectroscopy and dual polarization interferometry . The name was coined in 2003 by X. Han and J. Gross.

The lipidomics methods usually begin with an extraction of the lipids with a mixture of chloroform and methanol according to Bligh and Dyer from 1959. The lipids are then often separated by HPLC .

literature

• RW Gross: The evolution of lipidomics through space and time. In: Biochimica et Biophysica Acta . Volume 1862, number 8, 08 2017, pp. 731-739, doi : 10.1016 / j.bbalip.2017.04.006 , PMID 28457845 , PMC 5501277 (free full text).
• A. Triebl, J. Hartler, M. Trötzmüller, H. C Köfeler: Lipidomics : Prospects from a technological perspective. In: Biochimica et Biophysica Acta . Volume 1862, number 8, 08 2017, pp. 740-746, doi : 10.1016 / j.bbalip.2017.03.004 , PMID 28341148 .
• TA Lydic, YH Goo: Lipidomics unveils the complexity of the lipidome in metabolic diseases. In: Clinical and translational medicine. Volume 7, number 1, January 2018, p. 4, doi : 10.1186 / s40169-018-0182-9 , PMID 29374337 .

Individual evidence

1. ↑ H. Tsugawa, K. Ikeda, M. Arita: The importance of bioinformatics for connecting data-driven lipidomics and biological insights. In: Biochimica et Biophysica Acta . Volume 1862, number 8, 08 2017, pp. 762-765, doi : 10.1016 / j.bbalip.2017.05.006 , PMID 28514647 .
2. ^ AD Watson: Thematic review series: systems biology approaches to metabolic and cardiovascular disorders. Lipidomics: a global approach to lipid analysis in biological systems. In: Journal of lipid research. Volume 47, Number 10, October 2006, pp. 2101-2111, doi : 10.1194 / jlr.R600022-JLR200 , PMID 16902246 .
3. ↑ T. Hu, JL Zhang: Mass-spectrometry-based lipidomics. In: Journal of separation science. Volume 41, number 1, January 2018, pp. 351-372, doi : 10.1002 / jssc.201700709 , PMID 28859259 .
4. ^ X. Han, RW Gross: Global analyzes of cellular lipidomes directly from crude extracts of biological samples by ESI mass spectrometry: a bridge to lipidomics. In: Journal of lipid research. Volume 44, Number 6, June 2003, pp. 1071-1079, doi : 10.1194 / jlr.R300004-JLR200 , PMID 12671038 .
5. ^ EG Bligh, WJ Dyer: A rapid method of total lipid extraction and purification. In: Canadian journal of biochemistry and physiology. Volume 37, Number 8, August 1959, pp. 911-917, doi : 10.1139 / o59-099 , PMID 13671378 .
6. ^ MR Wenk: The emerging field of lipidomics. In: Nature reviews. Drug discovery. Volume 4, Number 7, July 2005, pp. 594-610, doi : 10.1038 / nrd1776 , PMID 16052242 .
7. ↑ K. Jurowski, K. Kochan, J. Walczak, M. Barańska, W. Piekoszewski, B. Buszewski: Analytical Techniques in Lipidomics: State of the Art. In: Critical reviews in analytical chemistry. Volume 47, number 5, September 2017, pp. 418-437, doi : 10.1080 / 10408347.2017.1310613 , PMID 28340309 .