MassTag-PCR

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MassTag PCR is a biochemical method, it is the combination of a polymerase chain reaction with mass spectrometry .

principle

The MassTag-PCR uses labeled primers in the polymerase chain reaction to amplify the DNA, mostly by multiplex PCR . The primers are marked by a UV-light- unstable bond. Following the PCR, the amplified DNA is separated from unbound primers by DNA extraction , whereby only markings from built-in primers are detected. The markings with different characteristic M / z ratios ( mass codes ) are released under UV light in the mass spectrometer . Compared to the multiplex qPCR , the MassTag-PCR has a higher detection limit .

Applications

The MassTag-PCR is used, among other things, to detect pathogens .

Individual evidence

  1. ^ A b T. Briese, G. Palacios, M. Kokoris, O. Jabado, Z. Liu, N. Renwick, V. Kapoor, I. Casas, F. Pozo, R. Limberger, P. Perez-Brena, J Ju, WI Lipkin: Diagnostic system for rapid and sensitive differential detection of pathogens. In: Emerging infectious diseases. Volume 11, Number 2, February 2005, pp. 310-313, ISSN  1080-6040 . doi : 10.3201 / eid1102.040492 . PMID 15752453 . PMC 3320438 (free full text).
  2. ↑ J. Doellinger, K. Schroeder, N. Witt, C. Heunemann, A. Nitsche: Comparison of real-time PCR and MassTag PCR for the multiplex detection of highly pathogenic agents. In: Molecular and cellular probes. Volume 26, Number 5, October 2012, pp. 177-181, ISSN  1096-1194 . doi : 10.1016 / j.mcp.2012.07.002 . PMID 22819946 .
  3. ↑ MR Wilson, KL Tyler: Issues and updates in emerging neurologic viral infections. In: Seminars in neurology. Volume 31, Number 3, July 2011, pp. 245-253, ISSN  1098-9021 . doi : 10.1055 / s-0031-1287650 . PMID 21964843 .
  4. ↑ SR Dominguez, T. Briese, G. Palacios, J. Hui, J. Villari, V. Kapoor, R. Tokarz, MP Glodé, MS Anderson, CC Robinson, KV Holmes, WI Lipkin: Multiplex MassTag-PCR for respiratory pathogens in pediatric nasopharyngeal washes negative by conventional diagnostic testing shows a high prevalence of viruses belonging to a newly recognized rhinovirus clade. In: Journal of clinical virology: the official publication of the Pan American Society for Clinical Virology. Volume 43, Number 2, October 2008, pp. 219-222, ISSN  1386-6532 . doi : 10.1016 / j.jcv.2008.06.007 . PMID 18674964 . PMC 2603178 (free full text).