Positional cloning

The positional cloning includes methods for identification of unknown DNA sequences in the immediate vicinity of a known DNA sequence. In contrast to functional cloning , positional cloning is based on the search for an adjacent DNA sequence on the same DNA molecule. This adjacent DNA sequence may belong to the same operon as the known sequence. The DNA is purified by DNA isolation and determined by primer walking (a method of DNA sequencing ).

Individual evidence

↑ TJ Aitman, E. Petretto, J. Behmoaras: Genetic mapping and positional cloning. In: Methods in molecular biology. Volume 597, 2010, pp. 13-32. doi : 10.1007 / 978-1-60327-389-3_2 . PMID 20013223 .
↑ E. Bortiri, D. Jackson, S. Hake: Advances in maize genomics: the emergence of positional cloning. In: Current opinion in plant biology. Volume 9, Number 2, April 2006, pp. 164-171. doi : 10.1016 / j.pbi.2006.01.006 . PMID 16458573 .
^ AY Deng: Positional cloning of quantitative trait Loci for blood pressure: how close are we ?: a critical perspective. In: Hypertension. Volume 49, Number 4, April 2007, pp. 740-747. doi : 10.1161 / 01.HYP.0000259105.09235.56 . PMID 17296871 . PDF .

[1] TJ Aitman, E. Petretto, J. Behmoaras: Genetic mapping and positional cloning. In: Methods in molecular biology. Volume 597, 2010, pp. 13-32. doi : 10.1007 / 978-1-60327-389-3_2 . PMID 20013223 .

[2] E. Bortiri, D. Jackson, S. Hake: Advances in maize genomics: the emergence of positional cloning. In: Current opinion in plant biology. Volume 9, Number 2, April 2006, pp. 164-171. doi : 10.1016 / j.pbi.2006.01.006 . PMID 16458573 .

[3] AY Deng: Positional cloning of quantitative trait Loci for blood pressure: how close are we ?: a critical perspective. In: Hypertension. Volume 49, Number 4, April 2007, pp. 740-747. doi : 10.1161 / 01.HYP.0000259105.09235.56 . PMID 17296871 . PDF .