Scintillation Proximity Assay

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The scintillation proximity assay (in German 'Scintillationsnähenachweis', SPA) is a biochemical method for the detection of protein-protein interactions .

principle

In the SPA, scintillator- filled microbeads are coated with a protein . The coated microbeads are then provided with a radioactively labeled protein solution, whereupon any binding partners that may be present bind to the coating. Due to the resulting proximity of the radioactive label to the scintillator and the beta decay , increased photons are released by the scintillator, which can be quantified photometrically . The SPA is used in drug design, among other things , and can be used in the course of high-throughput screening .

Individual evidence

  1. ↑ YW Park, RT Cummings, L. Wu, S. Zheng, PM Cameron, A. Woods, DM Zaller, AI Marcy, JD Hermes: Homogeneous proximity tyrosine kinase assays: scintillation proximity assay versus homogeneous time-resolved fluorescence. In: Analytical Biochemistry . Volume 269, Number 1, April 1999, ISSN  0003-2697 , pp. 94-104, doi : 10.1006 / abio.1999.4029 , PMID 10094779 .
  2. ^ S. Wu, B. Liu: Application of scintillation proximity assay in drug discovery. In: BioDrugs . Volume 19, Number 6, 2005, ISSN  1173-8804 , pp. 383-392, PMID 16392890 .
  3. ^ B. Liu, S. Li, J. Hu: Technological advances in high-throughput screening. In: American Journal of Pharmacogenomics . Volume 4, Number 4, 2004, ISSN  1175-2203 , pp. 263-276, PMID 15287820 .