Scorable marker
In genetic engineering, a scorable marker is a gene that is introduced into the modified organism together with the “gene of interest”, i.e. the gene that is actually desired, in order to be able to identify individuals who have successfully modified the gene. In contrast to selectable markers such as antibiotic resistance , scorable markers can be recognized directly from the phenotype of the genetically modified organism, for example by causing a certain color. Scorable markers are mainly used to select multicellular organisms.
application
Example of scorable markers in plants is the gene "uidA", which the acidic hydrolase β-glucuronidase encoded . This hydrolase enzymatically cleaves, for example, the dye X-Gluc to an insoluble blue CIBr indigo dye. This can be seen with the naked eye. Successful transformations of insects are indicated, for example, via the eye color, which is coded by the Scorable marker.
literature
- Alfred M. Handler, Anthony A. James: Insect transgenesis: methods and applications. CRC Press 2000, ISBN 0849320283
- George Acquaah: Principles of plant genetics and breeding. Wiley-Blackwell 2007, ISBN 9781405136464