Sperm-mediated gene transfer

The sperm-mediated gene transfer (engl. Sperm-mediated gene transfer , SMGT) is a method for gene therapy , which sperm as a vector used to generate a transgenic organism to produce. The sperm-mediated gene transfer combines artificial insemination through intracytoplasmic sperm injection (ICSI) with DNA- laden sperm.

principle

Sperm can bind and take up DNA in the head area (in the subacrosomal area and in the equatorial segment). For a sperm-mediated gene transfer, the semen is first removed in order to remove the inhibitory factor IF-1 . The efficiency of the uptake can be increased by an additional transfection . Restriction enzymes can increase the integration of transgenic DNA after fertilization of an egg. The sperm loaded with DNA can then be introduced into oocytes as part of artificial insemination by intracytoplasmic sperm injection . Since there is no insertion of the DNA into the DNA of the sperm, not all daughter cells are necessarily transgenic, but either completely transgenic, mosaic-like transgenic or, if the DNA is broken down prematurely , not transgenic.

Individual evidence

↑ Smith K, Spadafora C: Sperm-mediated gene transfer: applications and implications . In: Bioessays . 27, No. 5, May 2005, pp. 551-62. doi : 10.1002 / bies.20211 . PMID 15832378 .
↑ J. Parrington, K. Coward, J. Gadea: Sperm and testis mediated DNA transfer as a means of gene therapy. In: Syst Biol Reprod Med. (2011), Volume 57, Issue 1-2, pp. 35-42. doi : 10.3109 / 19396368.2010.514022 . PMID 21222517 . PDF .

[1] Smith K, Spadafora C: Sperm-mediated gene transfer: applications and implications . In: Bioessays . 27, No. 5, May 2005, pp. 551-62. doi : 10.1002 / bies.20211 . PMID 15832378 .

[2] J. Parrington, K. Coward, J. Gadea: Sperm and testis mediated DNA transfer as a means of gene therapy. In: Syst Biol Reprod Med. (2011), Volume 57, Issue 1-2, pp. 35-42. doi : 10.3109 / 19396368.2010.514022 . PMID 21222517 . PDF .