Partition chromatography

The partition is a chromatographic method, in which the stationary phase is always a liquid is, however, which is bound to a solid support material and thus immobile. The mobile phase can either also be a liquid (one then speaks of LLC , English: liquid liquid chromatography ), or a gas ( GLC , English: gas liquid chromatography ), see gas chromatography .

The chromatographic separation takes place through multiple distribution : the analyte is soluble to different degrees in the stationary and mobile phase. The better an analyte dissolves in the stationary phase, the longer it stays in it and the less far the mobile phase will transport it. Conversely, an analyte that is more soluble in the mobile phase will be transported a greater distance.

Partition chromatography was developed by Archer John Porter Martin together with Richard LM Synge . Both received the Nobel Prize in Chemistry in 1952 for their work .

A variant of partition chromatography is countercurrent partition chromatography , in which the stationary and mobile phases consist of two immiscible liquids.

Individual evidence

↑ Leslie S. Ettre: Milestones in Chromatography: The Birth of partition chromatography . (PDF). In: LCGC Europe. Volume 19, 2001, pp. 506-512.
^ Y. Ito: Development of Countercurrent Chromatography and Other Centrifugal Separation Methods. In: HJ Issaq (Ed.): A Century of Separation Science. Dekker, New York 2001, ISBN 0-8247-0576-9 , pp. 293-308.

[1] Leslie S. Ettre: Milestones in Chromatography: The Birth of partition chromatography . (PDF). In: LCGC Europe. Volume 19, 2001, pp. 506-512.

[2] Y. Ito: Development of Countercurrent Chromatography and Other Centrifugal Separation Methods. In: HJ Issaq (Ed.): A Century of Separation Science. Dekker, New York 2001, ISBN 0-8247-0576-9 , pp. 293-308.