Affibody

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Affibodies are artificial peptides derived from protein A from the bacterium Staphylococcus aureus , which are capable of binding antigens . They consist of 58 amino acids of the IgG binding domain of protein A, which are arranged in three α-helices . With a molecular mass of about 6  kDa , they are the smallest antigen-binding peptides next to Kunitz domain peptides. Affibodies are used in science in particular as an alternative to antibodies . In addition, Affibodies, such as the HER2 -specific ABY-025, are currently being clinically tested as potential cancer diagnostics .

properties

Because of their small molecular size, Affibodies have many advantageous physicochemical properties. These include high pH stability and heat tolerance. In addition, Affibodies have a superior tissue permeability compared to antibodies.

Manufacturing

Affibodies against different proteins can be synthesized by targeted or random changes of up to 13 of the 58 amino acids. Affibodies can also be conjugated with other molecules, such as dyes , and thus find further applications.

Individual evidence

  1. ↑ Gebauer M, Skerra A: Engineered protein scaffolds as next-generation antibody therapeutics . In: Curr Opin Chem Biol . June 2009. doi : 10.1016 / j.cbpa.2009.04.627 . PMID 19501012 .
  2. ↑ Tolmachev V: Imaging of HER-2 overexpression in tumors for guiding therapy . In: Curr. Pharm. Des. . 14, No. 28, 2008, pp. 2999-3019. doi : 10.2174 / 138161208786404290 . PMID 18991715 .
  3. ↑ Nord K, Gunneriusson E, Ringdahl J, Ståhl S, Uhlén M, Nygren PA: Binding proteins selected from combinatorial libraries of an alpha-helical bacterial receptor domain . In: Nat. Biotechnol. . 15, No. 8, August 1997, pp. 772-7. doi : 10.1038 / nbt0897-772 . PMID 9255793 .

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