Amplicon
In biochemistry, amplicon refers to a DNA sequence or RNA sequence that is duplicated by amplification .
properties
Amplicons are reproduced using various methods, for example by polymerase chain reaction or by ligase chain reaction . The product is called an amplificate. The length is determined by the distance between the primers . With shorter amplicons, the efficiency of the thermostable DNA polymerase used increases , which is why short amplicons are used in the qPCR .
literature
- Michael A. Innis: PCR Applications. Academic Press, 1999, ISBN 978-0-080-91963-8 .
Individual evidence
- ↑ John M. Walker: Molecular Biomethods Handbook. Springer Science & Business Media, 2008, ISBN 978-1-603-27375-6 , p. 150.
- ↑ Julie MJ Logan: Real-time PCR. Horizon Scientific Press, 2009, ISBN 978-1-904-45539-4 , p. 113.