HA tag

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The HA tag is a protein tag that is used in the generation and characterization of recombinant proteins .

properties

The HA tag is based on a part of hemagglutinin (position 98-106) and has the amino acid sequence Y P Y D V PDY A . It was published in 1988 by J. Field and colleagues and is one of the older protein tags. The monoclonal antibodies 12CA5 (mouse origin) and 3F10 (rat origin) bind to the HA tag. It can at N - or C -terminus of the protein is attached. It can be used up to three times in series to increase antibody binding. The HA tag is not suitable for the purification of proteins from apoptotic cells, since it is split off by the caspase-3 and caspase-7 according to the sequence DVPD.

Individual evidence

  1. ↑ a b J. Field, J. Nikawa, D. Broek, B. MacDonald, L. Rodgers, IA Wilson, RA Lerner, M. Wigler: Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. In: Molecular and cellular biology. Volume 8, Number 5, May 1988, pp. 2159-2165, PMID 2455217 , PMC 363397 (free full text).
  2. ^ Viki Allan: Protein Localization by Fluorescence Microscopy. OUP Oxford, 1999, ISBN 978-0-191-56591-5 , p. 187.
  3. ↑ a b Michael Wink: An Introduction to Molecular Biotechnology. John Wiley & Sons, 2006, ISBN 978-3-527-31412-6 , p. 217.
  4. ↑ L. Schembri, R. Dalibart, F. Tomasello, P. Legembre, F. Ichas, F. De Giorgi: The HA tag is cleaved and loses immunoreactivity during apoptosis. In: Nature methods. Volume 4, Number 2, February 2007, pp. 107-108, doi : 10.1038 / nmeth0207-107 , PMID 17264856 .