Cassette mutagenesis

The cassette mutagenesis is a method of targeted, site-specific production of mutations in a DNA molecule. It is conceptually the simplest method of targeted mutagenesis in molecular biology . It is based on the use of two oligodeoxynucleotide strands, usually synthetic , which are complementary to one another and which contain the desired mutation (s). Both strands are fused to form a short double-stranded DNA fragment, the mutagenesis cassette, and ligated into a DNA target molecule (mutagenesis frame) that has been cut with the aid of restriction enzymes . Because of this mechanism, cassette mutagenesis is used in particular for the complete replacement of smaller fragments of the encoded protein .

literature

• Wells JA, Vasser M., Powers DB: Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites . In: Genes . 34, No. 2-3, 1985, pp. 315-23. doi : 10.1016 / 0378-1119 (85) 90140-4 . PMID 3891521 .