Monocyte Activation Test

The monocyte activation test (MAT) is used to detect febrile substances in drugs.

The test simulates a natural immune response to a feverish reaction caused by a pyrogen . Human whole blood is used for the test. The test without animal testing offers the possibility of finding other fever-inducing substances in addition to pyrogens and endotoxins .

In 2010, the MAT was included in the European Pharmacopoeia as MAT monograph 2.6.30 and approved for the USA by the Food and Drug Administration two years later .

Web links

• Adaptation and establishment of the monocyte activation test (MAT) for implementation with bovine whole blood

Individual evidence

1. ↑ The monocyte activation test
2. ↑ In vitro pyrogen test Biospectrum
3. ↑ FDA: Guidance for Industry: Pyrogen and endotoxins testing: Questions and answers , June 2012 (PDF)