Northwestern Blot

The Northwestern Blot is a biochemical method for determining RNA-protein interactions .

principle

The North Western blotting combines separation of proteins by SDS-PAGE and transfer of proteins to a blotting membrane by Western blotting , followed by the Northern blot borrowed detection of RNA -binding proteins using RNA probes, previously radiolabeled were .

Web links

MeSH Blot Northwestern Blot

Individual evidence

↑ SK Lee, DL Hacker: In vitro analysis of an RNA binding site within the N-terminal 30 amino acids of the southern cowpea mosaic virus coat protein. In: Virology. Volume 286, Number 2, August 2001, pp. 317-327, doi : 10.1006 / viro.2001.0979 , PMID 11485399 .
↑ C. Franke, D. Gräfe, H. Bartsch, M. Bachmann: Use of non-radioactive detection method for north and southwestern blot. In: Methods in molecular biology (Clifton, NJ). Volume 536, 2009, pp. 441-449, doi : 10.1007 / 978-1-59745-542-8_44 , PMID 19378081 .
↑ Julio E Celis: Cell Biology. Academic Press, 2014, ISBN 978-1-483-28245-9 , p. 339.

[1] SK Lee, DL Hacker: In vitro analysis of an RNA binding site within the N-terminal 30 amino acids of the southern cowpea mosaic virus coat protein. In: Virology. Volume 286, Number 2, August 2001, pp. 317-327, doi : 10.1006 / viro.2001.0979 , PMID 11485399 .

[2] C. Franke, D. Gräfe, H. Bartsch, M. Bachmann: Use of non-radioactive detection method for north and southwestern blot. In: Methods in molecular biology (Clifton, NJ). Volume 536, 2009, pp. 441-449, doi : 10.1007 / 978-1-59745-542-8_44 , PMID 19378081 .

[books-GU-eBQAAQBAJ-515-3] Julio E Celis: Cell Biology. Academic Press, 2014, ISBN 978-1-483-28245-9 , p. 339.