Ribosome display

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The ribosome display is a biochemical method for detecting protein-protein interactions or for identifying the DNA sequence of a protein ligand . It is a variant of the molecular display .

principle

The addition of the antibiotic puromycin interrupts protein synthesis , during which the mRNA and the protein it encodes is fixed to the ribosome . Since the ribosome display takes place in vitro , it is also suitable for toxic proteins. Compared to the phage display , the ribosome display is faster, allows unnatural amino acids and larger RNA libraries. Furthermore, no cloning or transformation or transduction is required. Nucleases , among other things, pose problems .

Individual evidence

  1. ↑ a b A. Plückthun: Ribosome display: a perspective. In: Methods in molecular biology (Clifton, NJ). Volume 805, 2012, ISSN  1940-6029 , pp. 3-28, doi : 10.1007 / 978-1-61779-379-0_1 , PMID 22094797 .
  2. ↑ CG Ullman, L. Frigotto, RN Cooley: In vitro methods for peptide display and their applications. In: Briefings in functional genomics. Volume 10, number 3, May 2011, ISSN  2041-2657 , pp. 125-134, doi : 10.1093 / bfgp / elr010 , PMID 21628313 .
  3. ↑ T. Kanamori, Y. Fujino, T. Ueda: PURE ribosome display and its application in antibody technology. In: Biochimica et Biophysica Acta . Volume 1844, number 11, November 2014, ISSN  0006-3002 , pp. 1925-1932, doi : 10.1016 / j.bbapap.2014.04.007 , PMID 24747149 .