Ribotyping

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In molecular biology, ribotyping ( trunk word for ribosomal typing ) is a method for generating a genetic fingerprint of rDNA by means of restriction fragment length polymorphism .

principle

Characteristic restriction patterns of the DNA of genes of the ribosomal RNA (rRNA) are generated by ribotyping. The isolated DNA is broken down with a restriction enzyme , after agarose gel electrophoresis and a Southern blot with hybridization probes from parts of the 16S rRNA and 23 S rRNA genes, a pattern characteristic of the species can be generated and compared with samples from other species.

literature

Katharina Munk: pocket textbook biology microbiology. Thieme Verlag 2008. ISBN 978-3-13-144861-3 .

Individual evidence

^ F. Grimont, PA Grimont: Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. In: Ann. Inst. Pasteur Microbiol. (1986) Volume 137B, pp. 165-175. PMID 2446639 .
^ M. Pavlic, MW Griffiths: Principles, applications, and limitations of automated ribotyping as a rapid method in food safety. In: Foodborne Pathog Dis. (2009), Volume 6, No. 9, pp. 1047-1055. doi : 10.1089 / fpd.2009.0264 . PMID 19694556 .