Septin 9 test

The Septin-9 test is a blood test for the early detection of colon cancer . It is based on the detection of methylated Septin-9.

Procedure

It is known that tumors alter DNA methylation and thus influence the expression of genes . Septin-9 is one such gene that is more methylated in colon cancer . DNA can be isolated from a patient's blood sample. After bisulfite treatment it is possible either to sequence the DNA directly or to detect the target sequence using real time PCR . The Septin 9 test has also been commercially available since October 2009. The costs are currently not covered by the statutory health insurance.

Comparison with other prevention methods

The probability of correctly indicating an existing colon cancer ( sensitivity ) is given for the Septin 9 test with approx. 70% (instructions for use of the CE-marked test). This makes it better than the stool test for occult blood (gFOBT), which only finds colon cancer in 20–40% of cases. A special computed tomography of the large intestine, which has a sensitivity of approx. 65%, is better . The most accurate examination remains the colonoscopy , which finds over 95% of carcinomas in the intestine.

The specificity of the Septin 9 test is 90%, in contrast to the gFOBT with approx. 95% specificity.

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1. ↑ ^{a b c} Grützmann R, Molnar B, Pilarsky C, et al. : Sensitive detection of colorectal cancer in peripheral blood by septin 9 DNA methylation assay . In: PLoS ONE . 3, No. 11, 2008, p. E3759. doi : 10.1371 / journal.pone.0003759 . PMID 19018278 . PMC 2582436 (free full text).
2. ↑ Labor Krone: Septin 9 - A biomarker in the blood for colorectal cancer ( Memento from November 11, 2011 in the Internet Archive )
3. ↑ Muralidharan, MS Tint, AK Chadha, PP Chou and VM Pratt: Detecting colorectal cancer based on the presence of methylated septin 9 DNA in plasma. Archived from the original on February 24, 2016. Info: The archive link was inserted automatically and has not yet been checked. Please check the original and archive link according to the instructions and then remove this notice. In: Journal of Clinical Oncology . 28, 2010. Retrieved August 20, 2010. 
4. ↑ Allison JE, Tekawa IS, Ransom LJ, Adrain AL: A comparison of fecal occult-blood testing for colorectal-cancer screening. N Engl J Med . 1996 Jan 18; 334 (3): 155-9. PMID 8531970
5. ↑ Lieberman DA, Weiss DG; Veterans Affairs Cooperative Study Group 380: One-time screening for colorectal cancer with combined fecal occult-blood testing and examination of the distal colon. N Engl J Med. 2001 Aug 23; 345 (8): 555-60. PMID 11529208
6. ↑ Chaparro M, Gisbert JP, Del Campo L, Cantero J, Maté J: Accuracy of computed tomographic colonography for the detection of polyps and colorectal tumors: a systematic review and meta-analysis . In: Digestion . 80, No. 1, 2009, pp. 1-17. doi : 10.1159 / 000215387 . PMID 19407448 .
7. ^ Rex DK, Rahmani EY, Haseman JH, Lemmel GT, Kaster S, Buckley JS: Relative sensitivity of colonoscopy and barium enema for detection of colorectal cancer in clinical practice . In: Gastroenterology . 112, No. 1, January 1997, pp. 17-23. PMID 8978337 .

This article is about a health issue. It is not used for self-diagnosis and does not replace a diagnosis by a doctor. Please note the information on health issues !