Simulated body fluid

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A simulated body fluid (SBF) is a solution with an ion concentration similar to that of human blood plasma , whereby the same conditions prevail, such as the same pH value and the same physiological temperature. SBF was initially proposed by Kokubo et al. developed to evaluate the changes on a surface of a bioactive glass ceramic.

Applications

Surface modification of metallic implants

For artificial material to bond to living bones, the formation of an apatite layer on the surface of an implant is of great importance. SBF can be used as an in vitro test method to investigate the formation of the apatite layer on the surface of implants and thus to predict the in vivo bone bioactivity. The consumption of calcium and phosphate ions in the SBF solution leads to the spontaneous growth of bone-like apatite cores on the surface of biomaterials in vitro. This is why the formation of apatite on the surface of biological substances soaked in the SBF solution is considered a successful development of new bioactive materials. The SBF technique for changing the surface of metallic implants is usually a complex process, since it takes at least seven days to maintain uniform apatite layers on substrates with the daily refreshment of the SBF solution. Another method for reducing the coating time is to concentrate the calcium and phosphate ions in the SBF solution. Increased concentrations of calcium and phosphate ions in the SBF solution accelerate the coating process and meanwhile eliminate the need for regular replenishment of the SBF solution.

Gene therapy

An attempt was made to investigate the use of SBF in gene therapy . The calcium phosphate nanoparticles, required for the delivery of plasmid DNA (pDNA) in the nucleus of the cells, were synthesized in a SBF solution and mixed with pDNA. The in vitro studies showed higher gene delivery efficiency for the calcium-phosphate / DNA complexes produced by the SBF solution than those produced in pure water (for control).

Individual evidence

  1. ^ T. Kokubo: Bioactive glass ceramics: properties and applications. In: Biomaterials 12, 1991, pp. 155-163, doi : 10.1016 / 0142-9612 (91) 90194-F .
  2. ↑ T. Kokubo, H. Kushitani, S. Sakka, T. Kitsugi, T. Yamamuro: Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W In: Journal of Biomedical Materials Research 24, 1990, Pp. 721-734, doi : 10.1002 / jbm.820240607 .
  3. ↑ X. Chen, A. Nouri, Y. Li, J. Lin, PD Hodgson, C. Wen: Effect of Surface Roughness of Ti, Zr and TiZr on Apatite Precipitation from Simulated Body Fluid In: Biotechnology and Bioengineering 101, 2008, Pp. 378-387 doi : 10.1002 / bit.21900 .
  4. ↑ T. Kukubo, H. Takadama: How useful is SBF in predicting in vivo bone bioactivity? In: Biomaterials 27, 2006, pp. 2907-2915 doi : 10.1016 / j.biomaterials.2006.01.017 .
  5. ↑ P. Li, P. Ducheyne: Quasi-biological apatite film induced by titanium in a simulated body fluid In: Journal of Biomedical Materials Research 41, 1998, pp. 341-348, doi : 10.1002 / (SICI) 1097-4636 ( 19980905) 41: 3 <341 :: AID-JBM1> 3.0.CO; 2-C .
  6. ^ A. Nouri, R. Castro, JL Santos, C. Fernandes, J. Rodrigues, H. Tomás: Calcium phosphate-mediated gene delivery using simulated body fluid (SBF) In: International Journal of Pharmaceutics 434, 2012, p. 199 -208. doi : 10.1016 / j.ijpharm.2012.05.066 .