Trolox Equivalent Antioxidant Capacity

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The Trolox Equivalent Antioxidative Capacity ( TEAC ) can be used to indicate the antioxidant capacity of a sample. The vitamin E derivative Trolox serves as a reference for the measurement , which is why the result is given in Trolox equivalents. The TEAC value is often used when comparing samples containing polyphenols .

The principle of the measurement is based on the reaction of diammonium-2,2'-azino-di- (3-ethylbenzthiazoline) -6-sulfonate (ABTS) with the antioxidants contained in the medium to be examined . In an oxidative environment, ABTS forms a stable, green-colored radical cation that loses its color when it reacts with substances that have an antioxidant effect. By means of photometric measurements of the ABTS solution at 734 nm before and after a defined period of time (usually 6 minutes) after the addition of antioxidants, an absorbance difference is obtained, from the reduction in absorbance caused by Trolox in different concentrations, the antioxidative capacity the examined substance is determined.

literature

  • Miller, N. J .; Rice-Evans, C. et al. : "A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates". In: Clinical Science , 1993, 84 (4), pp. 407-412.
  • Re, R .; Pellegrini, N. et al. : "Antioxidant activity applying an improved ABTS radical cation decolorization assay." In: Free Radical Biology and Medicine , 1999, 26 (9-10), pp. 1231-1237.
  • Strube, M .; Haenen, GRMM, et al. : "Pitfalls in a Method for Assessment of Total Antioxidant Capacity." In: Free Radical Research , 1997 26 (6): pp. 515-521.
  • D. Huang, B. Ou, RL Prior: The chemistry behind antioxidant capacity assays. In: Journal of agricultural and food chemistry. Volume 53, Number 6, March 2005, pp. 1841-1856, doi : 10.1021 / jf030723c , PMID 15769103 .

Individual evidence

  1. ^ G. Cao, HM Alessio, RG Cutler: Oxygen-radical absorbance capacity assay for antioxidants. In: Free radical biology & medicine. Volume 14, Number 3, March 1993, pp. 303-311, PMID 8458588 .
  2. ↑ RL Prior, X. Wu, K. Schaich: Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. In: Journal of agricultural and food chemistry. Volume 53, Number 10, May 2005, pp. 4290-4302, doi : 10.1021 / jf0502698 , PMID 15884874 .