Van Gieson staining
The Van Gieson's Trichrome staining is a histological staining method which partially also as trichrome staining is used.
properties
Most of the variants of Van Gieson staining consist of a one-step staining with an aqueous, saturated picric acid solution and acid fuchsin . It is one of the picro fuchsine colors . It is used to color collagen and connective tissue , sometimes also in combination with elastic staining ( Elastika-Van Gieson staining ).
history
The Van Gieson staining was developed by Ira Van Gieson (1866–1913) in 1889 and was subsequently named after the inventor.
literature
- J. Bancroft, M. Gamble: Theory and Practice of Histological Techniques. 6th edition, Churchill-Livingstone, London 2008.
- B. Bricegirdle: A History of Microtechnique. 2nd edition, Science Heritage Ltd, Chicago 1986.
- FL Carson: Histotechnology A Self Instructional Text. 2nd edition, ASCP Press, Chicago 1997.
- D. Sheehan, BB Hrapchak: Theory and Practice of Histotechnology. 2nd edition, Mosby, St. Louis 1980.
Web links
- Bryan D. Llewellyn: Differential Staining With Acid Dyes. PDF .
Individual evidence
- ^ A b Ira van Gieson: Laboratory notes of technical methods for the nervous system. In: NY Med J. (1889), Volume 50, p. 57.
- ↑ Peter Gray: The Microtomist's Formulary and Guide. (1954). Originally from The Blakiston Co., later Robert E. Krieger Publishing Co.
- ^ I. Singh: A simple substitute of iron haematoxylin in the van Gieson and Masson trichrome procedures. In: Anatomischer Anzeiger . Volume 124, Number 5, 1969, ISSN 0003-2786 , pp. 490-491, PMID 4185232 .