Trichrome staining

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Dilated peritubular capillary vessels with sickle cells, stained according to Gömöri.
Rat lung tissue stained according to Masson. The connective tissue is blue, the cell nuclei dark red to purple and the cytoplasm red to pink.

The trichrome staining is a method for staining cells and tissues with three dyes (chromophores).

principle

The name trichrome is derived from the use of three dyes, for example the MSB trichrome stain ( Ponceau 6R together with Martinsgelb and water blue ). The specimens to be stained are fixed before histological staining , which stabilizes the structure of the cells and prevents autolysis . Various fixatives are used for this. B. a ten percent neutral buffered formaldehyde solution or the Bouin solution . Excess fixing agents are then washed out with a buffer if the staining requires this pretreatment.

variants

The variants of the trichrome staining are sorted chronologically.

Web links

Individual evidence

  1. ↑ KV Kanodia, AV Vanikar, KR Goplani, SB Gupta, HL Trivedi: Sickle cell nephropathy with diffuse proliferative lupus nephritis: a case report . In: Diagn Pathol . tape 3 , 2008, p. 9 , doi : 10.1186 / 1746-1596-3-9 , PMID 18307766 , PMC 2275218 (free full text).
  2. ↑ Ira van Gieson: Laboratory notes of technical methods for the nervous system. In: NY Med J. (1889), Volume 50, p. 57.
  3. ^ I. Singh: A simple substitute of iron haematoxylin in the van Gieson and Masson trichrome procedures. In: Anatomischer Anzeiger . Volume 124, Number 5, 1969, ISSN  0003-2786 , pp. 490-491. PMID 4185232 .
  4. ^ FB Mallory: A Contribution to staining methods. I. A differential stain for connective tissue fibril and reticulum. II. Chloride of Iron Haematoxylin for nuclei and fibrin. In: J Exp Med (1900), Volume 5, Issue 1, pp. 15-20. PMC 2117995 (free full text).
  5. ↑ Maria Wołuń-Cholewa, Krzysztof Szymanowski, Mirosław Andrusiewicz, Anna Szczerba, Jerzy B. Warchoł: Trichrome Mallory's stain may indicate differential rates of RNA synthesis in eutopic and ectopic endometrium. In: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society. Volume 48, Number 1, January 2010, ISSN  1897-5631 , pp. 148-152, doi: 10.2478 / v10042-008-0106-4 , PMID 20529831 .
  6. ^ P. Masson: Some histological methods; trichrome stainings and their preliminary technique. In: J. Tech. Methods. (1929), Vol. 12, pp. 75-90.
  7. ↑ J. Ouyang, M. Guzman, F. Desoto-Lapaix, MR Pincus, R. Wieczorek: Utility of desmin and a Masson's trichrome method to detect early acute myocardial infarction in autopsy tissues. In: International journal of clinical and experimental pathology. Volume 3, Number 1, 2009, ISSN  1936-2625 , pp. 98-105. PMID 19918333 , PMC 2776270 (free full text).
  8. ↑ Jacques Goldner: A modification of the masson trichrome technique for routine laboratory purposes. In: Am J Pathol. (1938), Volume 14, Issue 2, pp. 237-243. PMC 1964940 (free full text).
  9. ^ Y. Wang, P. Spencer: Evaluation of the interface between one-bottle adhesive systems and dentin by Goldner's trichrome. In: American journal of dentistry. Volume 18, Number 1, February 2005, ISSN  0894-8275 , pp. 66-72. PMID 15810485 .
  10. ^ RD Lillie: Studies on selective staining of collagen with acid aniline dyes. In: J. Tech. Meth. (1945), Vol. 25, pp. 1-47.
  11. ↑ G. Gömöri: A rapid one-step trichrome stain. In: American journal of clinical pathology. Volume 20, Number 7, July 1950, ISSN  0002-9173 , pp. 661-664. PMID 15432364 .
  12. ^ Y. Harati, K. Matta: Gomori's trichrome stain. In: Archives of neurology. Volume 36, Number 7, July 1979, ISSN  0003-9942 , pp. 454-455. PMID 88216 .
  13. ^ WB Wheatley: A rapid staining procedure for intestinal amoebae and flagellates. In: American journal of clinical pathology. Volume 21, Number 10, October 1951, ISSN  0002-9173 , pp. 990-991. PMID 14885115 .
  14. Jump up ↑ DJ Flournoy, SJ McNabb, ED Dodd, MH Shaffer: Rapid trichrome stain. In: Journal of clinical microbiology. Volume 16, Number 3, September 1982, ISSN  0095-1137 , pp. 573-574. PMID 6182159 , PMC 272416 (free full text).
  15. ↑ FM Salleh, TS Anuar, AM Yasin, N. Moktar: Wintergreen oil: a novel method in Wheatley's trichrome staining technique. In: Journal of microbiological methods. Volume 91, Number 1, October 2012, ISSN  1872-8359 , pp. 174-178, doi: 10.1016 / j.mimet.2012.08.004 , PMID 22986100 .
  16. ↑ G. Balogh, P. Kovács, G. Csaba: ASTRIN - a new trichrome staining technique. In: Journal for microscopic-anatomical research. Volume 96, Number 1, 1982, ISSN  0044-3107 , pp. 138-144. PMID 6179312 .
  17. ↑ M. Raica, O. Mederle, G. Raţ: A new trichrome staining method: its practice and application in normal tissues. In: Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie. Volume 44, Numbers 1-4, 1998 Jan-Dec, pp. 3-6, ISSN  1220-0522 . PMID 15678835 .