Wheatley trichrome stain

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The Wheatley trichrome staining , histological trichrome staining method .

properties

Most variants of Wheatley staining consist of a one-step staining in aqueous acetic acid solution with tungstophosphoric acid , Chromotrop 2R and Fast Green FCF . It is often combined with a hematoxylin stain beforehand. Wheatley trichrome stain is used to stain stool samples . The staining solution will degrade over time due to the low pH . Instead of Fast Green FCF, you can also dye with aniline blue or winter green oil .

history

Wheatley trichrome stain was developed by WB Wheatley in 1951. It is a further development of the Gömöri trichrome staining .

literature

  • J. Bancroft, M. Gamble: Theory and Practice of Histological Techniques. 6th edition, Churchill-Livingstone, London 2008.
  • B. Bricegirdle: A History of Microtechnique. 2nd edition, Science Heritage Ltd, Chicago 1986.
  • FL Carson: Histotechnology A Self Instructional Text. 2nd edition, ASCP Press, Chicago 1997.
  • D. Sheehan, BB Hrapchak: Theory and Practice of Histotechnology. 2nd edition, Mosby, St. Louis 1980.

Web links

  • Bryan D. Llewellyn: Differential Staining With Acid Dyes. PDF .

Individual evidence

  1. ^ A b W. B. Wheatley: A rapid staining procedure for intestinal amoebae and flagellates. In: American journal of clinical pathology. Volume 21, Number 10, October 1951, ISSN  0002-9173 , pp. 990-991, PMID 14885115 .
  2. Jump up ↑ DJ Flournoy, SJ McNabb, ED Dodd, MH Shaffer: Rapid trichrome stain. In: Journal of clinical microbiology. Volume 16, Number 3, September 1982, ISSN  0095-1137 , pp. 573-574, PMID 6182159 , PMC 272416 (free full text).
  3. ↑ FM Salleh, TS Anuar, AM Yasin, N. Moktar: Wintergreen oil: a novel method in Wheatley's trichrome staining technique. In: Journal of microbiological methods. Volume 91, Number 1, October 2012, ISSN  1872-8359 , pp. 174-178, doi : 10.1016 / j.mimet.2012.08.004 , PMID 22986100 .
  4. ↑ G. Gömöri: A rapid one-step trichrome stain. In: American journal of clinical pathology. Volume 20, Number 7, July 1950, ISSN  0002-9173 , pp. 661-664, PMID 15432364 .