Gömöri trichrome staining

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Dilated peritubular blood vessels with sickle cells , stained according to Gömöri.
Ragged Red Fibers in MELAS syndrome, colored with a Gömöri variant.

The gömöri trichrome stain is a histological trichrome staining method .

properties

Most of the variants of Gömöri staining consist of a single-stage staining in aqueous acetic acid solution with tungstophosphoric acid , Chromotrop 2R and Fast Green FCF . It is often combined with a hematoxylin stain beforehand. The gömöri trichrome stain is for coloring muscle - biopsies used. The staining solution will degrade over time due to the low pH . Instead of Fast Green FCF, aniline blue can also be used.

variants

The Masson trichrome stain uses acid fuchsin and Ponceau 2R as the red dye and light green SF as the green dye . The Masson-Goldner trichrome stain also uses orange G , but does not contain phosphotungstic acid. The Masson Trichrom & Verhoeff stain is a combination of stains that is used to stain blood vessels and to differentiate between arteries and veins , as the Verhoeff stain stains elastin black.

history

The Gömöri trichrome staining was developed in 1950 by György Gömöri (1904–1957). It is a further development of the Masson trichrome staining .

literature

  • J. Bancroft, M. Gamble: Theory and Practice of Histological Techniques. 6th edition. Churchill-Livingstone, London 2008.
  • B. Bricegirdle: A History of Microtechnique. 2nd Edition. Science Heritage, Chicago 1986.
  • FL Carson: Histotechnology A Self Instructional Text. 2nd Edition. ASCP Press, Chicago 1997.
  • D. Sheehan, BB Hrapchak: Theory and Practice of Histotechnology. 2nd Edition. Mosby, St. Louis 1980.

Web links

  • Bryan D. Llewellyn: Differential Staining With Acid Dyes. PDF .

Individual evidence

  1. ↑ KV Kanodia, AV Vanikar, KR Goplani, SB Gupta, HL Trivedi: Sickle cell nephropathy with diffuse proliferative lupus nephritis: a case report . In: Diagn Pathol . tape 3 , 2008, p. 9 , doi : 10.1186 / 1746-1596-3-9 , PMID 18307766 , PMC 2275218 (free full text) - ( diagnosticpathology.org ).
  2. ↑ a b G. Gömöri: A rapid one-step trichrome stain. In: American journal of clinical pathology. Volume 20, Number 7, July 1950, ISSN  0002-9173 , pp. 661-664. PMID 15432364 .
  3. ^ Y. Harati, K. Matta: Gomori's trichrome stain. In: Archives of neurology. Volume 36, Number 7, July 1979, ISSN  0003-9942 , pp. 454-455. PMID 88216 .
  4. ^ P. Masson: Some histological methods; trichrome stainings and their preliminary technique. In: J. Tech. Methods. (1929), Vol. 12, pp. 75-90.
  5. ↑ Jacques Goldner: A modification of the masson trichrome technique for routine laboratory purposes. In: Am J Pathol. (1938), Volume 14, Issue 2, pp. 237-243. PMC 1964940 (free full text).
  6. ^ Masson Trichrome, Verhoeff Stain. vetmed.vt.edu. URL: Archive link ( Memento of the original from September 27, 2011 in the Internet Archive ) Info: The archive link was inserted automatically and has not yet been checked. Please check the original and archive link according to the instructions and then remove this notice. . Accessed on: August 20, 2009. @1@ 2Template: Webachiv / IABot / education.vetmed.vt.edu
  7. ^ P. Masson: Some histological methods; trichrome stainings and their preliminary technique. In: J. Tech. Methods. (1929), Vol. 12, pp. 75-90.