Fast Green FCF

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Structural formula
Fast Green FCF structural formula
General
Surname Fast Green FCF
other names
  • Real green FCF
  • Food green 3
  • FD&C Green No. 3
  • Green 1724
  • Solid Green FCF
  • CI 42053
Molecular formula C 37 H 34 N 2 Na 2 O 10 S 3
Brief description

bluish-green solid

External identifiers / databases
CAS number 2353-45-9
EC number 219-091-5
ECHA InfoCard 100.017.356
PubChem 16888
ChemSpider 16002
Wikidata Q898299
properties
Molar mass 765.89 g mol −1
Physical state

firmly

Melting point

290 ° C (decomposition)

safety instructions
GHS labeling of hazardous substances
08 - Dangerous to health
H and P phrases H: 341
P: 281
As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

Fast Green FCF , also real green FCF , Food green 3 , FD&C Green No. 3 , Green 1724 , Solid Green FCF , and CI 42053 , is a bluish-green triphenylmethane dye , which is used outside the EU as food coloring E143 .

properties

Fast Green FCF as pure substance and in aqueous solution.

Fast Green FCF (from English for coloring food 'for food coloring') is used in histology as a substitute for light green SF in Masson's trichrome staining , as its color is stronger and less fading over time. Fast Green FCF is used to stain histones after DNA purification at alkaline pH . In agarose gel electrophoresis , Fast Green FCF is occasionally added to the sample buffer , where it is used to mark the solvent front. It is occasionally used for reversible protein staining in SDS-PAGE and Western Blot . The absorption maximum is at 620–625 nm.

Fast Green FCF is hardly absorbed in the intestine . Fast Green FCF is among other things not approved as a food additive in the EU. In the US, it is cans of peas and other green vegetables, jelly , sauces , fish , desserts and baked goods up to 100 mg / kg added.

In animal experiments were carcinogenic effects observed and mutagenic effects in cell cultures , experimental animals and humans. In higher concentrations it can cause irritation of the eyes, the skin, the digestive tract and the respiratory tract. Fast Green FCF has neurotoxic properties in cell cultures .

Individual evidence

  1. ↑ a b c d e Fast Green FCF data sheet , analytical standard at Sigma-Aldrich , accessed on May 15, 2017 ( PDF ).
  2. ↑ CM Wilson: Staining of proteins on gels: comparisons of dyes and procedures. In: Methods in enzymology. Volume 91, 1983, pp. 236-247, PMID 6190068 .
  3. ↑ CM Wilson: Studies and critique of Amido Black 10B, Coomassie Blue R, and Fast Green FCF as stains for proteins after polyacrylamide gel electrophoresis. In: Analytical biochemistry. Volume 96, Number 2, July 1979, pp. 263-278, PMID 89822 .
  4. ^ Joint FAO / WHO Expert Committee on Food Additives (JECFA), Monograph for Fast Green FCF , accessed December 9, 2014.
  5. ↑ Fast Green FCF, INS: 143 . Food and Agriculture Organization of the United Nations. Retrieved October 18, 2007.
  6. ↑ M. Ishidate, T. Sofuni, K. Yoshikawa, M. Hayashi, T. Nohmi, M. Sawada, A. Matsuoka: Primary mutagenicity screening of food additives currently used in Japan. In: Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association. Volume 22, Number 8, August 1984, pp. 623-636, PMID 6381265 .
  7. ↑ ML Hesselbach, RW O'Gara: Fast green- and light green-induced tumors: induction, morphology, and effect on host. In: Journal of the National Cancer Institute. Volume 24, April 1960, pp. 769-793, PMID 14401522 .
  8. ↑ Fast green FCF. In: IARC monographs on the evaluation of carcinogenic risk of chemicals to man. Volume 16, January 1978, pp. 187-197, PMID 640665 .
  9. ↑ Anonymous: Evaluation of certain food additives. In: World Health Organization technical report series. Number 952, 2009, pp. 1-208, 1 p following 208, PMID 20112497 .
  10. ^ WH Hansen, EL Long, KJ Davis, AA Nelson, OG Fitzhugh: Chronic toxicity of three food colors: Guinea Green B, Light Green SF Yellowish and Fast Green FCF in rats, dogs and mice. In: Food and cosmetics toxicology. Volume 4, Number 4, August 1966, pp. 389-410, PMID 5975245 .
  11. ↑ JA van Hooft: Fast Green FCF (Food Green 3) inhibits synaptic activity in rat hippocampal interneurons. In: Neuroscience letters. Volume 318, Number 3, February 2002, pp. 163-165, PMID 11803124 .

Web links

Commons : Fast Green FCF  - collection of images, videos and audio files