Mallory trichrome stain
The Mallory's trichrome stain is a histological trichrome staining method .
properties
Mallory's trichrome stain was used for differentiated staining of collagen and reticular fibers . After fixation, she uses acid fuchsine , molybdophosphoric acid or tungstophosphoric acid , orange G , aniline blue or methylene blue and oxalic acid for coloring . The cytoplasmic staining is done with acid fuchsin. The nucleic acids and protein fibers are stained with methylene blue. The addition of Orange G is used to color the erythrocytes .
history
Mallory trichrome staining was developed in 1900 by Frank Mallory (1862-1941).
literature
- J. Bancroft, M. Gamble: Theory and Practice of Histological Techniques. 6th edition, Churchill-Livingstone, London 2008.
- B. Bricegirdle: A History of Microtechnique. 2nd edition, Science Heritage Ltd, Chicago 1986.
- FL Carson: Histotechnology A Self Instructional Text. 2nd edition, ASCP Press, Chicago 1997.
- D. Sheehan, BB Hrapchak: Theory and Practice of Histotechnology. 2nd edition, Mosby, St. Louis 1980.
Web links
- Bryan D. Llewellyn: Differential Staining With Acid Dyes. PDF .
Individual evidence
- ^ A b F. B. Mallory: A Contribution to staining methods. I. A differential stain for connective tissue fibril and reticulum. II. Chloride of Iron Haematoxylin for nuclei and fibrin. In: J Exp Med. (1900), Volume 5, Issue 1, pp. 15-20. PMC 2117995 (free full text).