Acid Blue 22

Structural formula
General
Surname	Acid Blue 22
other names	Disodium 2-amino-3-methyl-5 - {[4- (4-sulfonatoanilino) phenyl] - [4- (4-sulfophenyl) iminocyclohexa-2,5-dien-1-ylidene] methyl} benzenesulfonate ( IUPAC ) ; CI 42755; Aniline blue soluble in water; Water blue; Pure blue; China blue;
Molecular formula	C 32 H 25 N 3 Na 2 O 9 S 3
Brief description	odorless, dark blue-brown powder
External identifiers / databases
EC number	249-113-9
ECHA InfoCard	100.044.634
PubChem	135871623
ChemSpider	21173243
Wikidata	Q421881
properties
Molar mass	737.74 g mol −1
Physical state	firmly
solubility	soluble in water, poorly in ethanol
safety instructions
GHS labeling of hazardous substances	no GHS pictograms
	no GHS pictograms
H and P phrases	H: no H-phrases
	P: no P-phrases
	As far as possible and customary, SI units are used. Unless otherwise noted, the data given apply to standard conditions .

CI Acid Blue 22 (also water-soluble aniline blue ) is an acid dye from the group of triphenylmethane dyes . Both the substance mixture of Acid Blue 22 with the very similar Acid Blue 93 ( methyl blue ) and these two pure substances themselves are often referred to as water-soluble aniline blue .

properties

Acid Blue 22 has an absorption maximum in the wavelength range of 600 nm. The dye is soluble in water.

use

Acid Blue 22 stains collagen in tissue structures blue. It is used as dye for the Polychrom - staining of histological sections, in the cytology and the pH - indicator used (pH 10.0 to 13.0). It is also used to make chromosomes visible. It is also used for the manufacture of ink and the dyeing of cotton, silk and paper. The dye is also contained in China Blue Lactose Agar and serves as a pH indicator to show the acid formation by bacteria when lactose is broken down.

Individual evidence

↑ ^a ^b data sheet at OMIKRON GmbH , accessed on December 27, 2019.
↑ ^a ^b entry on water blue. In: Römpp Online . Georg Thieme Verlag, accessed on July 25, 2012.
↑ ^a ^b Electron Microscopy Sciences: Safety Data Sheet Aniline Blue, Certified CI 42780 , accessed December 27, 2019.
↑ Aniline blue dyes , accessed December 27, 2019.
↑ ^a ^b Data sheet Aniline blue, CI 42755, Certified, Water-soluble at Polysciences, Inc , accessed on March 8, 2015.
↑ Eckhard Bast: Microbiological Methods: An Introduction to Basic Working Techniques . 2nd Edition. Spectrum Akademischer Verlag GmbH, Heidelberg / Berlin 2001, ISBN 978-3-8274-1072-6 , p. 48-50 .

[omikron-1] ta sheet at OMIKRON GmbH , accessed on December 27, 2019.

[roempp-online-2] try on water blue. In: Römpp Online . Georg Thieme Verlag, accessed on July 25, 2012.

[emsdiasum-3] Electron Microscopy Sciences: Safety Data Sheet Aniline Blue, Certified CI 42780 , accessed December 27, 2019.

[aeisner-4] Aniline blue dyes , accessed December 27, 2019.

[polysciences-5] Data sheet Aniline blue, CI 42755, Certified, Water-soluble at Polysciences, Inc , accessed on March 8, 2015.

[bast-6] Eckhard Bast: Microbiological Methods: An Introduction to Basic Working Techniques . 2nd Edition. Spectrum Akademischer Verlag GmbH, Heidelberg / Berlin 2001, ISBN 978-3-8274-1072-6 , p. 48-50 .