Migration electrophoresis

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The migration electrophoresis (also countercurrent electrophoresis - English called crossover electrophoresis ) is a detection technique that allows to examine blood or other body fluids with small sample amounts . The method is based on the immunoelectrophoretic evaluation of an antigen-antibody reaction.

description

Migration electrophoresis uses the different migration speeds of antigen and antibody as well as antigen-antibody complexes in electrophoresis . If one component migrates over the other, reactions can occur. If the serum is applied in the middle between the antigen and the antibody, its components migrate in one direction or the other during electrophoresis. Where antibody and antigen meet, a precipitate appears at the point of interaction. If the reaction appears on the side of the antibody application, the serum contains antigens. If the precipitation is on the side of the antigen application, the serum contains antibodies.

application

The method developed in the 1950s is suitable for a wide range of medical examinations. For example, rapid tests for infections such as hepatitis can be carried out quickly and cheaply. Migration electrophoresis was also used for basic scientific research.

In archeology , it can be used to identify those animal species that were hunted by humans using stone projectiles and devices that go back to the Paleolithic . Often blood has penetrated into the finest hairline cracks in the stone objects and preserved in the tiny crevices by sharpening the edges . For example, human blood was found at the suspected end of the handle of the largest fluted projectile tip from the Clovis culture .

literature

  • J. Haan, N. Lang: Quantitative determination of the antigen binding in the antigen-antibody complex with migration electrophoresis . In: Klinische Wochenschrift , 1957, 35, pp. 1013-1014. doi: 10.1007 / BF01488725
  • KH Gillich: Quantitative determination of the binding of 131 I-thyroxine by serum proteins using migration electrophoresis . In: Journal for the whole of experimental medicine , 1958, 130, p. 415. doi: 10.1007 / BF02046123
  • C. Vergani: Crossover electrophoresis for the rapid detection of serum hepatitis (Australia) antigen and antibody. In: Journal of Clinical Pathology , 1971, 24, pp. 86-87, PMC 478035 (free full text)
  • Axel Schulze-Thulin: Indians of the prehistoric times Diedrichs, Munich 1995, ISBN 3-424-01209-2
  • Emil Hoffmann: Lexicon of Archeology . 1999 ISBN 3-406-42125-3 , p. 78.

Individual evidence

  1. ↑ Spektrum.de, Lexicon of Biochemistry
  2. ↑ Crossover electrophoresis for the rapid detection of serum hepatitis (Australia) antigen and antibody . PMC 478035 (free full text)
  3. ^ W. Gerlich: Laboratory diagnosis of the hepatitis B virus infection . (PDF)
  4. ↑ Quantitative determination of the binding of 131 I-thyroxine by serum proteins using migration electrophoresis