Agglutination polymerase chain reaction

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The agglutination PCR (ADAP) is a biochemical method to determine the amount of antibodies . It is a variant of the polymerase chain reaction (PCR), more precisely the immuno-PCR .

properties

The agglutination PCR uses the antigen of the antibodies to be quantified, which has been previously marked with primers . The binding of antibodies to the antigen creates immune complexes that agglutinate with one another . As with other immuno-PCR, agglutination PCR combines the specific binding of antibodies to (any) antigens with the sensitivity of PCR. As a result, the detection limit is in the zeptomolar range with a measurement range five powers of ten above.

Individual evidence

  1. ↑ Cheng-Ting Tsai, Peter V. Robinson, Carole A. Spencer, Carolyn R. Bertozzi: Ultrasensitive Antibody Detection by Agglutination-PCR (ADAP) . In: ACS Central Science . 2, No. 3, 2016, pp. 139–147. doi : 10.1021 / acscentsci.5b00340 . PMID 27064772 . PMC 4819452 (free full text).
  2. ↑ Le Chang, Jinming Li, Lunan Wang: Immuno-PCR: An ultrasensitive immunoassay for biomolecular detection . In: Analytica Chimica Acta . 910, 2016, pp. 12-24. doi : 10.1016 / j.aca.2015.12.039 . PMID 26873464 .
  3. ↑ Mark Spengler, Michael Adler, Christof M. Niemeyer: Highly sensitive ligand-binding assays in pre-clinical and clinical applications: Immuno-PCR and other emerging techniques . In: The Analyst . 140, No. 18, 2015, pp. 6175-6195. doi : 10.1039 / C5AN00822K . PMID 26196036 .
  4. ↑ Feng Shen, Wenbin Du, Jason E. Kreutz, Alice Fok, Rustem F. Ismagilov: Digital PCR on a Slip Chip . In: Lab on a Chip . 10, No. 20, 2010, pp. 2666-2672. doi : 10.1039 / c004521g . PMID 20596567 . PMC 2948063 (free full text).
  5. ↑ Jesus Rodriguez-Manzano, Mikhail A. Karymov, Stefano Begolo, David A. Selck, Dmitriy V. Zhukov, Erik Jue, Rustem F. Ismagilov: Reading Out Single-Molecule Digital RNA and DNA Isothermal Amplification in Nanoliter Volumes with Unmodified Camera Phones . In: ACS Nano . 10, No. 3, 2016, pp. 3102-3113. doi : 10.1021 / acsnano.5b07338 . PMID 26900709 .