Boyden Chamber
The Boyden chamber is a cell culture vessel that is used in cell biology . The chamber consists of two compartments which are separated by a membrane with pores about 5 to 10 µm in size. The Boyden chamber is mainly used in chemotaxis experiments. A mixture of a cell culture medium and the substance to be tested is introduced into one compartment, and cells in the cell culture medium into the other compartment. Diffusion creates a concentration gradient in the pores of the membrane , which the cells try to follow. After an incubation time that depends on the test conditions , the cells are fixed and the number of cells that have passed the membrane is counted.
The Boyden Chamber was developed by Stephen Boyden at the Australian National University in Canberra and first published in August 1962.
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- ↑ Boyden SV: The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. . In: J Exp Med . 115, 1962, pp. 453-66. PMID 13872176 .