Dische rehearsal

The Dische test (or Dische test) is a specific detection reaction for deoxyribose and thus u. a. for the distinction between RNA and DNA , named after its discoverer Zacharias Dische (1895–1988).

Execution and response

The Dische reagent used for the sample is a sulfuric acid solution of diphenylamine in glacial acetic acid . It turns blue when heated with an aqueous deoxyribose solution. So far nothing definite is known about the structure of the dye itself, but that deoxyribose first breaks down under the action of strong mineral acids with dehydration and ring opening to ω-hydroxy-levulinaldehyde (4-oxo-5-hydroxy-pentanal), which then breaks down with the Diphenylamine provides the blue color.

Earlier assumptions, according to which the Dische sample would run over furfural that had formed in the meantime , have not been confirmed.

See also

• Bial sample
• Molisch sample
• Selivanov rehearsal

Individual evidence

1. ^ Wilhelm Flörke, Robert Wolff: Course themes chemistry . Dümmler Verlag, Bonn 1982, pp. 6-10, ISBN 3-427-43121-5 .
2. ↑ Jennifer Patterson, Cameron Mura: Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples ; Journal of Visualized Experiments ; 2013; (72): 50225. PMC 3597041 (free full text).

Web links

• Ruhr University Bochum: Basic biochemical internship, experiment number G-10: Properties of nucleic acids .