Dulbecco's Modified Eagle's Medium
Dulbecco's Modified Eagle's Medium , DMEM for short , is a standardized nutrient medium for cell culture with a wide range of uses for human and various animal cells. It's a variant of Eagle's Minimum Essential Medium (EMEM) , but it contains more nutrients than this. It was named after Renato Dulbecco and Harry Eagle .
composition
Information in mg / l:
Inorganic salts:
- CaCl 2 (anhydrous): 200.00
- Fe (NO 3 ) 3 • 9 H 2 O: 0.10
- KCl : 400.00
- MgSO 4 (anhydrous): 97.67
- NaCl : 6400.00
- NaH 2 PO 4 • H 2 O: 125.00
- NaHCO 3 : 3700.00
Other components:
- D -glucose: 4500.00
- Phenol red : 15.00
- Sodium pyruvate: 110.00
- L-arginine HCl: 84.00
- L-cystine 2HCl: 63.00
- L-glutamine: 584.00
- Glycine: 30.00
- L-histidine HCl H 2 O: 42.00
- L-isoleucine: 105.00
- L-leucine: 105.00
- L-lysine HCl: 146.00
- L-methionine: 30.00
- L-phenylalanine: 66.00
- L-serine: 42.00
- L-threonine: 95.00
- L-tryptophan: 16.00
- L-Tyrosine 2Na · 2H 2 O: 104.33
- L-valine: 94.00
Vitamins :
- D-calcium pantothenate: 4.00
- Choline chloride: 4.00
- Folic acid 4.00
- i-inositol 7.20
- Niacinamide 4.00
- Riboflavin 0.40
- Thiamine HCl 4.00