Endo agar
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Endo agar
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E. coli on endo agar with a clearly visible fuchsine sheen
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Endo agar (also lactose fuchsin sulfite agar), developed by the Japanese bacteriologist Shigeru Endo (1869–1937), is a selective medium that is used in microbiology for the cultivation of enterobacteria , especially coliform bacteria . These utilize the lactose they contain and produce aldehydes and organic acids. The aldehydes release fuchsine from the fuchsine sulfite complex and color the colonies from deep purple to reddish. Colonies of Escherichia coli also show a characteristic yellowish-green sheen when the fuchsin crystallizes (fuchsin sheen). Gram-positive bacteria are largely prevented from growing by sodium sulfite and fuchsine. The slightly violet to pink color of the nutrient medium is characteristic . By oxidation of the sulfite it is more intensely colored reddish and unusable. To avoid loss of quality, it should always be stored in the dark.
Individual evidence
- ↑ S. Endo: About a method for the detection of typhus bacilli. In: Centralbl. Bakt. I. Orig. 35, 1904, pp. 109-110.
- ↑ Microbiology manual . Merck KGaA , Darmstadt 2007, OCLC 271427237 , p. 278 ( analytics-shop.com [PDF]). Microbiology manual ( Memento of the original dated February 16, 2017 in the Internet Archive ) Info: The archive link was inserted automatically and has not yet been checked. Please check the original and archive link according to the instructions and then remove this notice.
