FOX reagent

The FOX reagent is a solution for the detection of hydroperoxides in biological material.

principle

The FOX reagent consists of an aqueous solution of 0.25 mM ammonium iron (II) sulfate , 100 mM sorbitol , 25 mM sulfuric acid and 0.1 mM xylenol orange . The sample is diluted 1:20 with the FOX reagent and incubated for half an hour at room temperature. In the presence of hydroperoxides, the Fe ^{2+ is} oxidized to Fe ³⁺ and then forms a dye complex with xylenol orange, which is measured photometrically at a wavelength of 560 nm. The FOX reagent is used, among other things, to measure lipid hydroperoxides in liposomes (with a modified protocol). The FOX2 reagent is a further development of the FOX reagent.

literature

Jaffar Nourooz-Zadeh: Gas chromatography-mass spectrometry assay for measurement of plasma isoprostanes. In: Methods in Enzymology. Volume 300, 1999, pp. 13-17. doi: 10.1016 / S0076-6879 (99) 00107-X .

Individual evidence

↑ ZY Jiang, JV Hunt, SP Wolff: Ferrous ion oxidation in the presence of xylenol orange for detection of lipid hydroperoxide in low density lipoprotein. In: Analytical biochemistry. 202, number 2, May 1992, pp. 384-389, PMID 1519766 .
↑ ^a ^b T P Labuza: Maillard Reactions in Chemistry, Food and Health. Elsevier, 1998, ISBN 978-1-84569-839-3 , p. 330.
↑ ^a ^b Vladimir Torchilin: Liposomes: A Practical Approach. OUP Oxford, 2003, ISBN 978-0-19-963654-9 , p. 49.
↑ Jacob Zabicky: Analytical and safety aspects of hydroperoxide and related functional groups . In: Zvi Rappoport (Ed.): The Chemistry of Peroxides, Parts 1 and 2. John Wiley & Sons, 2006, ISBN 978-0-470-86275-9 , p. 676 ( limited preview in Google book search).
↑ J. Nourooz-Zadeh, J. Tajaddini-Sarmadi, KL Ling, SP Wolff: Low-density lipoprotein is the major carrier of lipid hydroperoxides in plasma. Relevance to determination of total plasma lipid hydroperoxide concentrations. In: The Biochemical journal. Volume 313 (Pt 3), February 1996, pp. 781-786, PMID 8611155 , PMC 1216978 (free full text).

[1] ZY Jiang, JV Hunt, SP Wolff: Ferrous ion oxidation in the presence of xylenol orange for detection of lipid hydroperoxide in low density lipoprotein. In: Analytical biochemistry. 202, number 2, May 1992, pp. 384-389, PMID 1519766 .

[Labuza-2] T P Labuza: Maillard Reactions in Chemistry, Food and Health. Elsevier, 1998, ISBN 978-1-84569-839-3 , p. 330.

[Torchilin-3] Vladimir Torchilin: Liposomes: A Practical Approach. OUP Oxford, 2003, ISBN 978-0-19-963654-9 , p. 49.

[Zvi_Rappoport-4] Jacob Zabicky: Analytical and safety aspects of hydroperoxide and related functional groups . In: Zvi Rappoport (Ed.): The Chemistry of Peroxides, Parts 1 and 2. John Wiley & Sons, 2006, ISBN 978-0-470-86275-9 , p. 676 ( limited preview in Google book search).

[5] J. Nourooz-Zadeh, J. Tajaddini-Sarmadi, KL Ling, SP Wolff: Low-density lipoprotein is the major carrier of lipid hydroperoxides in plasma. Relevance to determination of total plasma lipid hydroperoxide concentrations. In: The Biochemical journal. Volume 313 (Pt 3), February 1996, pp. 781-786, PMID 8611155 , PMC 1216978 (free full text).