Feulgen reaction

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Feulgen reaction in the salivary glands of deer tick virus infected ticks.

The proof of Feulgen and Ross Beck , or just the Feulgen reaction is one of Joachim Wilhelm Robert Feulgen co-developed 1924 histochemical method for the detection of deoxyribonucleic acid (DNA). First, the DNA in the sample to be analyzed (tissue sample, sample of microorganisms ) is hydrolyzed by hydrochloric acid , whereby the molecular compounds of base and sugar are separated. Free aldehyde groups are formed . With fuchsin sulphurous acid ( Schiff's reagent ), the aldehydes turn red-violet in color. These DNA residues can now be recognized under the microscope . Optionally, it can be counter-colored with light green SF .

Individual evidence

  1. ↑ Entry on Feulgen staining. In: Römpp Online . Georg Thieme Verlag, accessed on June 8, 2014.