Fusion protein
A fusion protein (also hybrid protein ) is created through the joint expression of two genes or gene parts that lie one behind the other in the genome . By removing the stop codon after the first gene or by amalgamating it through a chromosome change (e.g. a translocation ), both genes are read as if they were a single gene.
A well-known fusion protein that can arise naturally through a translocation is the BCR-ABL gene product, which is considered to be the cause of chronic myeloid leukemia (CML). The resulting shortened chromosome 22 is known as the Philadelphia chromosome .
In biochemistry fusion proteins artificially produced are often used in order as part of a protein engineering for. B. to examine the subcellular localization of a certain gene product or to find a protein more easily ( protein tag ). Fluorescent proteins are often used as so-called reporter proteins, which are then fused at the C- or N-terminal point with the protein to be examined. The term fusion protein is sometimes used for a fusogenic protein .
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- Jan Murken, Tiemo Grimm, and Elke Holinski-Feder; Human genetics; ISBN 978-3-13-139297-8