Gal4 / UAS system
Using the GAL4 / UAS system (also UAS-GAL4 system ) can any cloned gene selectively in specific cells or tissues expressing be. GAL4 is a yeast-specific transcription factor that is under the control of a weak promoter and codes for the GAL4 protein. Another promoter or enhancer is required for expression . The GAL4 protein binds specifically to the so-called UAS (Upstream Activating Sequence), which activates a target gene located downstream. This can e.g. B. the gene for the green fluorescent protein GFP , or a gene to be examined.
The GAL4 / UAS system was developed by Andrea Brand and Norbert Perrimon at Harvard Medical School for the model organism Drosophila melanogaster and first published in Development in 1993 . It is often used in conjunction with molecular biology methods such as the Enhancer Trap method.
literature
- ↑ Chris B. Phelps, Andrea H. Brand: Ectopic Gene Expression inDrosophilaUsing GAL4 System. In: Methods. 14, 1998, pp. 367-379, doi : 10.1006 / meth.1998.0592 .
- ^ Brand, Perrimon (1993). Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development, Vol. 118, Issue 2 pp. 401-415