Semiconductor sequencing
Semiconductor sequencing is a method for DNA sequencing in which released protons are detected with a semiconductor during replication .
principle
During the replication of the DNA, nucleoside triphosphates are incorporated into the DNA, with diphosphate ( pyrophosphate ) being split off by hydrolysis . The released diphosphate receives a newly won hydroxyl group , which gives off a proton that is registered with an ion-sensitive field effect transistor . All four possible bases are added one after the other; if protons are released, the next nucleotide in the DNA corresponds to the one added last. Repetitions of the same nucleotide can be identified based on the intensity of the measured signal. The name is intended to make it clear that no photometric methods are used to read out the information, such as in pyrosequencing and the installation of integrated circuits similar to computer chips, a continuous increase in speed and cost reduction through improved production techniques ( Moore's law ).
Individual evidence
- ↑ Hildegard Kaulen: Semiconductor sequencing - the espresso machine of genome researchers . FAZ.NET , August 9, 2011
- ↑ Jonathan M. Rothberg et al. a .: An integrated semiconductor device enabling non-optical genome sequencing . In: Nature . tape 475 , no. 7356 , July 21, 2011, p. 348–352 , doi : 10.1038 / nature10242 ( nature.com [PDF]).