Immobilized cell

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An immobilized cell is a cell that has been immobilized . Immobilized cells are used as biocatalysts .

properties

Immobilized cells are an alternative to purified and immobilized enzymes and are used when purification of the enzyme is problematic or several successive enzymatic reactions are necessary. The immobilized cells are mostly used in a bioreactor with continuous liquid flow. The substances used for immobilization are partly similar to those from 3D cell culture . For this purpose, the reaction products must be isolated from the culture medium in the case of living immobilized cells , in contrast to isolation from a buffer in the case of dead immobilized cells. In comparison to immobilized enzymes , immobilized cells are often less expensive because there is no protein purification to isolate the enzyme. However, other enzymes are also present in immobilized cells, which can result in more undesirable reaction products.

literature

  • G. Kumar, A. Mudhoo, P. Sivagurunathan, D. Nagarajan, A. Ghimire, CH Lay, CY Lin, DJ Lee, JS Chang: Recent insights into the cell immobilization technology applied for dark fermentative hydrogen production. In: Bioresource Technology . Volume 219, November 2016, pp. 725-737, doi : 10.1016 / j.biortech.2016.08.065 , PMID 27561626 .
  • Jose M. Guisan: Immobilization of Enzymes and Cells. Springer Science & Business Media, 2006, ISBN 978-1-597-45053-9 .
  • Viktor Nedovic: Applications of Cell Immobilization Biotechnology. Springer Science & Business Media, 2006, ISBN 978-1-402-03363-6 , p. 316.

Individual evidence

  1. ^ V. Nedović, B. Gibson, TF Mantzouridou, B. Bugarski, V. Djordjević, A. Kalušević, A. Paraskevopoulou, M. Sandell, D. Šmogrovičová, M. Yilmaztekin: Aroma formation by immobilized yeast cells in fermentation processes. In: Yeast. Volume 32, Number 1, January 2015, pp. 173-216, doi : 10.1002 / yea.3042 , PMID 25267117 .
  2. ^ Rene H. Wijffels: Immobilized Cells. Springer Science & Business Media, 2012, ISBN 978-3-642-56891-6 , p. 45.
  3. ^ D. Thomas, T. O'Brien, A. Pandit: Toward Customized Extracellular Niche Engineering: Progress in Cell-Entrapment Technologies. In: Advanced materials. Volume 30, number 1, January 2018, p., Doi : 10.1002 / adma.201703948 , PMID 29194781 .
  4. ↑ CM Kisukuri, LH Andrade: Production of chiral compounds using immobilized cells as a source of biocatalysts. In: Organic & biomolecular chemistry. Volume 13, Number 40, October 2015, pp. 10086-10107, doi : 10.1039 / c5ob01677k , PMID 26366634 .