Knock-in

from Wikipedia, the free encyclopedia

In genetics, knock-in (also known as gene knock-in ) refers to processes for the targeted insertion of a transgene , ie a gene that did not previously exist in a genome or of new or modified DNA sequences.

principle

The transgene is usually inserted into a specific gene locus in the genome of cell lines or model organisms such as mice. In contrast to the classic production of genetically modified organisms , the insertion is sequence-specific, which means that there is less interference in gene expression through insertion in transcription-active areas of the DNA . By means of a gene knockin, genes can be specifically inserted or inactivated by specific insertions and then activated again by removing the insertion using a recombinase . The gene knockin can also inactivate genes by inserting them into promoters , which is used to investigate the functions of unknown genes or as a model for diseases based on genetic defects .

Individual evidence

  1. ^ Greg Gibson: A Primer Of Genome Science. , 2nd edition, Sunderland, 2004. ISBN 0-87893-232-1 . P. 308.
  2. ↑ JF Mata, T. Lopes, R. Gardner, LE Jansen: A rapid FACS-based strategy to isolate human gene knockin and knockout clones. In: PloS one. Volume 7, number 2, 2012, p. E32646, ISSN  1932-6203 . doi : 10.1371 / journal.pone.0032646 . PMID 22393430 . PMC 3290580 (free full text).
  3. ↑ J. Dunkelberger, L. Zhou, T. Miwa, WC Song: C5aR expression in a novel GFP reporter gene knockin mouse: implications for the mechanism of action of C5aR signaling in T cell immunity. In: Journal of Immunology (Baltimore, Md.: 1950). Volume 188, Number 8, April 2012, pp. 4032-4042, ISSN  1550-6606 . doi : 10.4049 / jimmunol.1103141 . PMID 22430734 . PMC 3324670 (free full text).
  4. ^ BC Cox, Z. Liu, MM Lagarde, J. Zuo: Conditional gene expression in the mouse inner ear using Cre-loxP. In: Journal of the Association for Research in Otolaryngology: JARO. Volume 13, Number 3, June 2012, pp. 295-322, ISSN  1438-7573 . doi : 10.1007 / s10162-012-0324-5 . PMID 22526732 . PMC 3346893 (free full text).
  5. ^ A. Doyle, MP McGarry, NA Lee, JJ Lee: The construction of transgenic and gene knockout / knockin mouse models of human disease. In: Transgenic research. Volume 21, Number 2, April 2012, pp. 327-349, ISSN  1573-9368 . doi : 10.1007 / s11248-011-9537-3 . PMID 21800101 . PMC 3516403 (free full text).