Moeller staining

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The Moeller stain is a histological staining for endospores . It is a method of endospore staining .

principle

First, the bacteria are placed on a slide and fixed by heating . The Moeller stain uses carbol fuchsin to stain endospores and methylene blue as a counterstain . Alternatively, Schaeffer-Fulton staining with malachite green and safranine counterstaining or Dorner-Snyder staining with carbol fuchsin and nigrosine counterstaining is used. A variant of Moeller staining was developed for staining endospores without heating.

Individual evidence

  1. ^ RW Fairbrother, Geoffrey A. Taylor: A Text-Book of Bacteriology. 9th edition, Butterworth-Heinemann, 2014. ISBN 9781483195575 . P. 473.
  2. ↑ a b M. Hayama, K. Oana, T. Kozakai, S. Umeda, J. Fujimoto, H. Ota, Y. Kawakami: Proposal of a simplified technique for staining bacterial spores without applying heat – successful modification of Moeller's method. In: European journal of medical research. Volume 12, Number 8, August 2007, ISSN  0949-2321 , pp. 356-359, PMID 17933713 .
  3. ↑ DA Mormak, LE Casida: Study of Bacillus subtilis Endospores in Soil by Use of a Modified Endospore Stain. In: Applied and environmental microbiology. Volume 49, Number 6, June 1985, ISSN  0099-2240 , pp. 1356-1360, PMID 16346801 , PMC 241728 (free full text).