Murashige-Skoog medium

Mammillaria spp. on MS0 medium in an Erlenmeyer flask

The Murashige-Skoog medium , also MSO or MS0 ( MS-zero ), is a plant nutrient medium that is used in laboratories for the cultivation of plant tissue cultures . It was developed by Toshio Murashige and Folke K. Skoog in search of a new plant growth regulator. It is the most common medium in plant tissue culture experiments.

Skoog's PhD student Murashige was supposed to discover a growth hormone in tobacco juice, but it did not succeed. Instead, analyzes of pressed tobacco and ashed tobacco found higher concentrations of certain minerals in plant tissues than previously assumed. After a few experiments it was shown that the variation in the levels of these nutrients, especially nitrogen , produced increased growth.

literature

• Toshio Murashige, Folke Skoog: A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures . In: Physiologia Plantarum , Jg. 15 (1962), No. 3, pp. 473-497, ISSN  0031-9317 , doi : 10.1111 / j.1399-3054.1962.tb08052.x

Web links

• Overview of different culture media: MK Razdan: Introduction to Plant Tissue Culture. Science Publishers, 2003, ISBN 978-1-578-08237-7 , p. 22 ( limited preview in Google book search).